After verification, an anti-doublecortin antibody recommended for free-floating application on mouse brain tissue is not currently available. However, a citation on AB2253 for free-floating application on mouse brain tissue was found and may be of interest for reference.
Product: AB2253 Sigma-Aldrich Anti-Doublecortin Antibody
Publication: Nat Commun. 2015 Mar 26;6:6606
Title: Aberrant hippocampal neurogenesis contributes to epilepsy and associated cognitive decline
PMID: 25808087
价格与库存信息目前不能提供
推荐产品
生物来源
guinea pig
质量水平
抗体形式
serum
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
rat, mouse
种属反应性（根据同源性预测）
human (based on 100% sequence homology)
技术
immunofluorescence: suitable (Frozen tissue sections)
immunohistochemistry: suitable (Paraffin)
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... DCDC1(341019)
一般描述
抗双皮质素抗体，目录号AB2253，是检测双皮质素的豚鼠多克隆抗体，经测试可用于免疫组化（石蜡）、免疫荧光和蛋白质印迹中。
Target molecular weight ~42 kDa observed;40.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
特异性
与其他物种的反应性尚未确定。
这种豚鼠多克隆抗体可检测鼠和大鼠物种中的双皮质素。它靶向的是一个在C末端区域含有17个氨基酸的表位。
免疫原
表位：C端
重组肽。
应用
抗双皮质素抗体已被用于免疫组化和免疫荧光染色中。
生化/生理作用
Doublecortin(DCX)是神经发生的重要标志物。它是研究和比较不同物种的神经发生过程的宝贵工具。[1]
质量
通过蛋白质印迹在大鼠脑组织裂解液中进行了评估。
蛋白印迹分析：该抗体的1：1,000稀释液可在大鼠脑组织裂解液中检测到双皮质素。
蛋白印迹分析：该抗体的1：1,000稀释液可在大鼠脑组织裂解液中检测到双皮质素。
目标描述
观察分子量为〜45 kDa；计算分子量为40.57 kDa。在某些裂解物中可以观察到未鉴定的条带。
联系
替代品：AB5910
外形
GST去除豚鼠血清，含0.05%叠氮化钠
GST耗尽
储存及稳定性
自收到之日起在-20°C可稳定保存1年。使用建议：收到后，在取下瓶盖之前，将小瓶离心并轻轻混合溶液。分装到微量离心管中，并储存于 -20°C。避免反复冻融循环，以免损坏 IgG 和影响产品性能。
分析说明
对照
大鼠脑裂解液。
大鼠脑裂解液。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
其他客户在看
Jacques Balthazart et al.
Brain, behavior and evolution, 84(1), 1-4 (2014-07-19)
Commentary on “Evaluating the Predictive Value of Doublecortin as a Marker for Adult Neurogenesis in Canaries (Serinus canaria)” by Michiel Vellema, Moritz Hertel, Susan L. Urbanus, Annemie Van der Linden, and Manfred Gahr (Journal of Comparative Neurology 2014, 522 :
Embryonic Nkx2. 1-expressing neural precursor cells contribute to the regional heterogeneity of adult V-SVZ neural stem cells
Delgado RN and Lim DA
Developmental Biology, 407(2), 265-274 (2015)
Tomás P Bachor et al.
Neurobiology of disease, 108, 13-28 (2017-07-27)
Diabetes mellitus (DM) is reaching epidemic conditions worldwide and increases the risk for cognition impairment and dementia. Here, we postulated that progenitors in adult neurogenic niches might be particularly vulnerable. Therefore, we evaluated the different components of the mouse subventricular
Adolescent Changes in Cellular Proliferation in the Dentate Gyrus of Male and Female C57BL/6N Mice Are Resilient to Chronic Oral Corticosterone Treatments
Shome A, et al.
Frontiers in Behavioral Neuroscience, 12, 192-192 (2018)
Tania Campos-Ordoñez et al.
Experimental neurology, 261, 236-244 (2014-05-27)
Hydrocephalus can develop secondarily to a disturbance in production, flow and/or absorption of cerebrospinal fluid. Experimental models of hydrocephalus, especially subacute and chronic hydrocephalus, are few and limited, and the effects of hydrocephalus on the subventricular zone are unclear. The
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Is it possible to obtain a small aliquot of specific antibodies for testing purposes to determine their suitability for staining Doublecortin (DCX) in immunohistochemistry on free-floating mouse brain tissue fixed with PFA? The antibodies of interest are the guinea pig polyclonal and the goat polyclonal.
1 answer-
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