The maleimide group in this linker reacts with a free thiol to form a thiosuccinimide, as illustrated by the mechanism provided in the link below.
After conjugating to a thiol-containing protein, it is important to consider the encapsulation or functionalization methods for a liposome. This application necessitates a review of existing scientific literature to develop this application. Below is some information that should be helpful to you that discusses this in more detail, describing postinsertion methods and strategies for adsorbing onto vector surfaces.
