During Quality Control, no testing to performed involving confocal microscopy. Conjugate 5-aminofluorescein (AFL)-human serum albumin has been used to stain brain cells, although it does not appear confocal microscopy was performed. Below is the reference: https://www.sigmaaldrich.com/tech-docs/paper/250406
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表单
powder
mp
223 °C (dec.) (lit.)
溶解性
methanol: 5 mg/mL
λmax
496 nm
应用
diagnostic assay manufacturing
hematology
histology
储存温度
room temp
SMILES字符串
Nc1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35
InChI
1S/C20H13NO5/c21-10-1-4-14-13(7-10)19(24)26-20(14)15-5-2-11(22)8-17(15)25-18-9-12(23)3-6-16(18)20/h1-9,22-23H,21H2
InChI key
GZAJOEGTZDUSKS-UHFFFAOYSA-N
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一般描述
荧光胺，异构体I属于衍生荧光素类。
应用
荧光胺，异构体 I 适用于特异性、灵敏的亚硝酸盐分光光度测定法。它已被用于通过竞争性羧基-胺偶联反应进行纳米颗粒的荧光标记，以显示纳米颗粒的内部情况。
警示用语：
Warning
危险分类
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
J T Jones et al.
Molecular and biochemical parasitology, 30(1), 35-44 (1988-07-01)
We have studied the characteristics of binding of the polycation poly-L-lysine to the schistosome surface. Two consequences of this binding were measured: (a) tegumental damage, as assessed by the uptake of the DNA binding stain Hoechst 33258, and (b) the
S Miyauchi et al.
Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, 12(1), 27-34 (1996-01-01)
The protective efficacy of sodium hyaluronate (Na-HA) on the corneal endothelium against the damage induced by sonication was investigated using enucleated rabbit eyes and Na-HA fluorescence labeled with 5-aminofluorescein (FA-HA). The anterior chamber was reformed by injecting a 1% solution
F Schwesinger et al.
Proceedings of the National Academy of Sciences of the United States of America, 97(18), 9972-9977 (2000-08-30)
Point mutants of three unrelated antifluorescein antibodies were constructed to obtain nine different single-chain Fv fragments, whose on-rates, off-rates, and equilibrium binding affinities were determined in solution. Additionally, activation energies for unbinding were estimated from the temperature dependence of the
P Gribbon et al.
Biophysical journal, 77(4), 2210-2216 (1999-10-08)
Hyaluronan (HA) is a highly hydrated polyanion, which is a network-forming and space-filling component in the extracellular matrix of animal tissues. Confocal fluorescence recovery after photobleaching (confocal-FRAP) was used to investigate intramolecular hydrogen bonding and electrostatic interactions in hyaluronan solutions.
Surface modification of cell culture carriers: routes to anhydride functionalization of polystyrene.
Mirko Nitschke et al.
Colloids and surfaces. B, Biointerfaces, 90, 41-47 (2011-10-22)
Physico-chemical and topographical cues allow to control the behavior of adherent cells. Towards this goal, commercially available cell culture carriers can be finished with a laterally microstructured biomolecular functionalization. As shown in a previous study [Biomacromolecules 4 (2003) 1072], the
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Can this be used to stain cells and visualize them under the confocal microscope?
1 answer-
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Dear manager, I want to ask you 2 questions about this product. 1. Is it a flurescence dye? Can you show me its adsorption or emission spectrum? 2. Is the amine group reactive?
1 answer-
Yes, Fluoresceinamine, isomer I is classified as belonging to a group of derivatized fluorosceins. The product is fluorescent. Although no quality control testing is performed to check for excitation and emission, the dye is expected to excite around 490 nm and emit around 520 nm.
As the H2N- group is not acetylated or protonated, the amine group can be considered reactive. There is literature available stating Fluoresceinamine, isomer I can be covalently bound to human serum albumin.
Helpful?
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What is the solubility of Fluoresceinamine, isomer I, CAS 3326-34-9?
1 answer-
Soluble in methanol at a concentration of 5 mg/mL. Soluble in DMSO at a concentration of ≥ 32 mg/mL.
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