11333089001
Roche
Anti-Digoxigenin
from sheep
Synonyme(s) :
anti-digoxigenin, digoxigenin
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About This Item
Code UNSPSC :
12352203
Produits recommandés
Source biologique
sheep
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
polyclonal
Forme
lyophilized
Conditionnement
pkg of 200 μg
Fabricant/nom de marque
Roche
Isotype
IgG
Température de stockage
2-8°C
Description générale
Digoxigenin is a hapten which is used in labeling of nucleic acids and in detection systems.
Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes. It allows faster detection, is less hazardous and has an increased shelf life.
Spécificité
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
Application
Anti-Digoxigenin has been used in DNA tethering. It has been used to attach DNA molecule to the glass surface of the flow cell.
Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled compounds using:
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Western blot
Actions biochimiques/physiologiques
In the presence of Na+, Mg2+ and ATP, digoxigenin inhibits sodium pumps.
Notes préparatoires
Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
Working solution: For coating applications: PBS (phosphate buffered saline), pH 7.4
- ELISA: for coating: 2 to 4 μg/ml
- Immunohistocytochemistry: 0.5 to 2 μg/ml
- In situ hybridization: 0.2 to 0.4 μg/ml
- Western blot: 0.5 to 2 μg/ml
Working solution: For coating applications: PBS (phosphate buffered saline), pH 7.4
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption.
Reconstitution
Add 1 ml PBS to a final concentration of 200 μg/ml.
Remarque sur l'analyse
No cross reactivity with other steroids, such as human estrogens e.g., estradiol or androgens e.g., testosterone.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
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Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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