11333062910
Roche
Anti-Digoxigenin
from mouse IgG1κ (clone 1.71.256)
Synonyme(s) :
anti-digoxigenin, digoxigenin
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About This Item
Code UNSPSC :
12352203
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
clone 1.71.256, monoclonal
Forme
lyophilized
Conditionnement
pkg of 100 μg
Fabricant/nom de marque
Roche
Technique(s)
ELISA: 2-4 μg/mL
immunocytochemistry: 0.5-2 μg/mL
immunohistochemistry: 0.5-2 μg/mL
western blot: 0.5-2 μg/mL
Isotype
IgG1κ
Température de stockage
2-8°C
Description générale
Contents: Lyophilizate
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life.
Spécificité
The monoclonal antibody reacts with free and bound digoxigenin.
Application
Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled componds using:
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Western blot
- Immunofluorescence staining
- FISH (fluorescent in situ hybridization)
Actions biochimiques/physiologiques
In the presence of Na+, Mg2+ and adenosine triphosphate (ATP), digoxigenin inhibits sodium pumps.
Caractéristiques et avantages
- Specific to free and bound digoxigenin
- The antibody is not stabilized with protein and therefore suitable as coating antibody in Sandwich-ELISAs and for labeling procedures. For example it can be conjugated with enzymes or fluorescence dyes for direct detection of digoxigenin-labeled samples.
Notes préparatoires
Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
Working solution: For coating applications
phosphate buffered saline, pH 7.4
- ELISA: for coating: 2 to 4 μg/ml
- Immunohistocytochemistry: 0.5 to 2 μg/ml
- In situ hybridization: 0.2 to 0.4 μg/ml
- Western blot: 0.5 to 2 μg/ml
Working solution: For coating applications
phosphate buffered saline, pH 7.4
Reconstitution
Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
Stockage et stabilité
The lyophilized antibody is stable at +2 to +8°C. The reconstituted antibody solution is stable up to 6 months at +2 to +8°C. The solution can be stored in aliquots at -15 to -25°C. Avoid repeated freezing and thawing.
Remarque sur l'analyse
There are no cross reactivities known.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
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Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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