11207733910
Roche
Anti-Digoxigenin-POD, Fab fragments
from sheep
Synonyme(s) :
anti-digoxigenin, digoxigenin
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
12352203
Produits recommandés
Source biologique
sheep
Niveau de qualité
Conjugué
peroxidase conjugate
Forme d'anticorps
F(ab′)2 fragment of affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Forme
lyophilized (clear, brown solution after reconstitution)
Conditionnement
pkg of 150 U
Fabricant/nom de marque
Roche
Température de stockage
2-8°C
Description générale
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. This product contains Fab fragments from an anti-digoxigenin antibody, conjugated with horse-radish peroxidase (POD). Anti-Digoxigenin-POD, Fab fragments is useful for the detection of digoxigenin-labeled compounds.
Spécificité
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
Application
Use Anti-Digoxigenin-POD, Fab fragments for the detection of digoxigenin-labeled compounds using:
- Dot blot
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Western blot
Notes préparatoires
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.
Working concentration: Working concentration of conjugate will depend on the application and substrate. The following concentrations should be taken as a guideline: Dot blot: 150 mU/ml
Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
Working concentration: Working concentration of conjugate will depend on the application and substrate. The following concentrations should be taken as a guideline: Dot blot: 150 mU/ml
- ELISA: 50 to 150 mU/ml
- Immunohistocytochemistry: 250 to 500 mU/ml
- In situ hybridization: 1.5 to 7.5 U/ml
- Southern blot: 150 mU/ml
- Western blot: 250 to 500 mU/ml
Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
Reconstitution
Add 1 ml double-distilled water to a final concentration of 150 U/ml.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Vous ne trouvez pas le bon produit ?
Essayez notre Outil de sélection de produits.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Yutaka Daido et al.
Developmental biology, 392(1), 117-129 (2014-05-07)
The vertebrate retina contains two types of photoreceptor cells, rods and cones, which use distinct types of opsins and phototransduction proteins. Cones can be further divided into several subtypes with differing wavelength sensitivity and morphology. Although photoreceptor development has been
Sarah E Mercer et al.
PloS one, 7(12), e52375-e52375 (2013-01-10)
The inability to functionally repair tissues that are lost as a consequence of disease or injury remains a significant challenge for regenerative medicine. The molecular and cellular processes involved in complete restoration of tissue architecture and function are expected to
Juha Kotimaa et al.
Molecular immunology, 76, 13-21 (2016-06-24)
Experimental mouse models have been extensively used to elucidate the role of the complement system in different diseases and injuries. Contribution of gender has revealed an intriguing gender specific difference; female mice often show protection against most complement driven injuries
Hande Tunbak et al.
eLife, 9 (2020-05-06)
The zebrafish was used to assess the impact of social isolation on behaviour and brain function. As in humans and other social species, early social deprivation reduced social preference in juvenile zebrafish. Whole-brain functional maps of anti-social isolated (lonely) fish
X Peng et al.
Leukemia, 29(12), 2355-2365 (2015-06-25)
Controlled self-renewal and differentiation of hematopoietic stem/progenitor cells (HSPCs) are critical for vertebrate development and survival. These processes are tightly regulated by the transcription factors, signaling molecules and epigenetic factors. Impaired regulations of their function could result in hematological malignancies.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique