Direkt zum Inhalt
Merck

Characterization of Metabolic Patterns in Mouse Oocytes during Meiotic Maturation.

Molecular cell (2020-10-18)
Ling Li, Shuai Zhu, Wenjie Shu, Yueshuai Guo, Yusheng Guan, Juan Zeng, Haichao Wang, Longsen Han, Jiaqi Zhang, Xiaohui Liu, Chunling Li, Xiaojing Hou, Min Gao, Juan Ge, Chao Ren, Hao Zhang, Tim Schedl, Xuejiang Guo, Minjian Chen, Qiang Wang
ZUSAMMENFASSUNG

Well-balanced and timed metabolism is essential for making a high-quality egg. However, the metabolic framework that supports oocyte development remains poorly understood. Here, we obtained the temporal metabolome profiles of mouse oocytes during in vivo maturation by isolating large number of cells at key stages. In parallel, quantitative proteomic analyses were conducted to bolster the metabolomic data, synergistically depicting the global metabolic patterns in oocytes. In particular, we discovered the metabolic features during meiotic maturation, such as the fall in polyunsaturated fatty acids (PUFAs) level and the active serine-glycine-one-carbon (SGOC) pathway. Using functional approaches, we further identified the key targets mediating the action of PUFA arachidonic acid (ARA) on meiotic maturation and demonstrated the control of epigenetic marks in maturing oocytes by SGOC network. Our data serve as a broad resource on the dynamics occurring in metabolome and proteome during oocyte maturation.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Monoklonales Anti-β-Aktin in Maus hergestellte Antikörper, clone AC-15, ascites fluid
Sigma-Aldrich
All-trans-Retinal, powder, ≥98%
Sigma-Aldrich
Pyridoxal 5′-Phosphat Hydrat, ≥98%
Sigma-Aldrich
D-Glucuronsäure, ≥98% (GC)
Sigma-Aldrich
Taurocholsäure Natriumsalz Hydrat, ≥95% (HPLC)
Sigma-Aldrich
cis-4,7,10,13,16,19-Docosahexaensäure, ≥98%
Sigma-Aldrich
Albumin aus Rinderserum, lyophilized powder, low endotoxin, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)
Sigma-Aldrich
Inosin, ≥99% (HPLC)
Sigma-Aldrich
5-Aminolevulinsäure -hydrochlorid, ≥98%
Sigma-Aldrich
β-Alanin, 99%
Sigma-Aldrich
DL-Glyceraldehyd, ≥90% (GC)
Sigma-Aldrich
D-Panthenol, ≥98.0% (NT)
Sigma-Aldrich
Arachidonsäure, from non-animal source, ≥98.5% (GC)
Sigma-Aldrich
Dodecandisäure, 99%
Sigma-Aldrich
Hypotaurin, ≥98% (TLC)
Sigma-Aldrich
2′-Desoxycytidin, ≥99% (HPLC)
Sigma-Aldrich
5′-Deoxy-5′-(methylthio)adenosin
Sigma-Aldrich
Histamin -dihydrochlorid, ≥99.0% (AT)
Sigma-Aldrich
D-Saccharinsäure Kaliumsalz, ≥98% (GC)
Sigma-Aldrich
L-Rhamnose, natural sourced, 99%, FG
Sigma-Aldrich
L-Norvalin, arginase inhibitor
Sigma-Aldrich
D-(−)-Chinasäure, 98%
Sigma-Aldrich
Orotsäure, ≥98% (titration), anhydrous
Sigma-Aldrich
Agmatin -sulfat (Salz), ≥97%, powder
Sigma-Aldrich
4-Methylcatechin, ≥95%
Sigma-Aldrich
S-(5′-Adenosyl)-L-Methionin -p-toluolsulfonat (Salz), ≥80% (HPLC), ≥80% (spectrophotometric assay)
Sigma-Aldrich
Anti-α-Tubulin−-FITC-Antikörper, monoklonaler Antikörper der Maus, clone DM1A, purified from hybridoma cell culture
Sigma-Aldrich
DL-5-Hydroxy-lysin -hydrochlorid
Sigma-Aldrich
Glycocholsäure Hydrat, synthetic, ≥97% (HPLC)
Sigma-Aldrich
Asp-Phe, 96%