U0880

Uricase from Candida sp.

recombinant, expressed in E. coli, lyophilized powder, ≥2 units/mg solid

• Synonym(e): Urate: oxygen oxoreductase
• CAS-Nummer: 9002-12-4
• EC-Nummer: 1.7.3.3 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00082126
• UNSPSC-Code: 12352204
• PubChem Substanz-ID: 24900619
• NACRES: NA.54

Eigenschaften

• Rekombinant: expressed in E. coli
• Qualitätsniveau: 200
• Form: lyophilized powder
• Spezifische Aktivität: ≥2 units/mg solid
• Mol-Gew.: 35 kDa by SDS-PAGE
• Lagertemp.: −20°C
• SMILES String: [P](=O)([O-])(OC3C(OC(C3O)N4C=CC(=O)NC4=O)CO)OCC1OC(C(C1O)O)N2C=CC(=O)NC2=O.[N+H4]
• InChI: 1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3
• InChIKey: VPXDHZMLJOJKOX-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Uricase deficient mice develop uric acid nephropathy and diabetes insipidus which can be mitigated with recombinant uricase treatment.

Uricase is a homo-tetrameric protein with its active sites at the head-to-tail monomer-dimer interface.

Anwendung

Uricase from Candida sp. has been used:

• to eliminate urate in plasma samples and evaluate the contribution of urate to the total plasma antioxidant capacity
• to intraperitoneally inject the mice to study the role of uric acid in fibrin extracellular traps (ET) formation
• for elimination of uric acid in ferric reducing/antioxidative power (FRAP) assay to measure the total antioxidative capacity of human plasma sample; in uricase-based biosensors

Biochem./physiol. Wirkung

Uricase participates in purine catabolism. It catalyzes the conversion of highly insoluble uric acid into 5-hydroxyisourate. Accumulation of uric acid causes liver/kidney damage or chronically causes gout. In mice, a mutation in the gene encoding uricase causes a sudden increase in uric acid. Mice, deficient in this gene, exhibit hyperuricemia, hyperuricosuria, and uric acid crystalline obstructive nephropathy.

Einheitendefinition

One unit will oxidize 1.0 μmole of uric acid to allantoin per minute at pH 8.5 at 25 °C.

Physikalische Form

Powder; contains citrate and sucrose

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable