T1063
Thrombin aus Humanplasma
lyophilized powder, ≥2800 NIH units/mg protein (E1%/280, 18.3)
Synonym(e):
Faktor IIa
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About This Item
Empfohlene Produkte
Form
lyophilized powder
Qualitätsniveau
Spezifische Aktivität
≥2800 NIH units/mg protein (E1%/280, 18.3)
Mol-Gew.
37.4 kDa
Verunreinigungen
HIV, hepatitis B and hepatitis C, tested negative
UniProt-Hinterlegungsnummer
Anwendung(en)
diagnostic assay manufacturing
Lagertemp.
−20°C
Angaben zum Gen
human ... F2(2147)
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Allgemeine Beschreibung
Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.
Anwendung
Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to evaluate the in vitro effect of low molecular weight heparin on thrombin generation in cirrhotic patients at different stages of liver disease.
Biochem./physiol. Wirkung
Serinprotease, die selektiv Arg-Gly-Bindungen in Fibrinogen spaltet, um Fibrin und Fibrinopeptide A und B zu bilden.
Einheitendefinition
Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard
Physikalische Form
Lyophilisiert aus 0.02 M Bis/Tris Puffer, pH 6.5, 0.15 M NaCl und 0.1% PEG-8000
Hinweis zur Analyse
The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.
Sonstige Hinweise
Für weitere Informationen siehe thrombin at www.sigma-aldrich.com/enzymeexplorer.
Haftungsausschluss
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Inhibitor
Produkt-Nr.
Beschreibung
Preisangaben
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Zielorgane
Respiratory system
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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