SRP6270

MMP-2 human

recombinant, expressed in HEK 293 cells, ≥92% (SDS-PAGE)

• Synonym(e): CLG4, CLG4A, MMP-II, MMP2, MONA, TBE-1
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in HEK 293 cells
• Markierung: 6-His tagged (C-terminus)
• Assay: ≥92% (SDS-PAGE)
• Form: lyophilized powder
• Mol-Gew.: calculated mol wt 71.8 kDa; observed mol wt 66-71 kDa (DTT-reduced. Ala 30 is the predicted N-terminus.)
• Verpackung: pkg of 10 and 50 μg
• Verunreinigungen: <1 EU/μg endotoxin (LAL test)
• UniProt-Hinterlegungsnummer: P08253
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: human ... MMP-2(4313)

Beschreibung

Allgemeine Beschreibung

Matrix metalloproteinase-2 (MMP-2) which is also known as 72kDa type IV collagenase, 72kDa gelatinase, gelatinase A and CLG4A, belongs to the peptidase M10A family. It contains three fibronectin type-II domains and four hemopexin-like domains. MMP-2 is produced by normal skin fibroblasts. The gene encoding this protein is localized on human chromosome 16q12.2.

Biochem./physiol. Wirkung

Matrix metalloproteinase-2 (MMP-2) cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln. It is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. It degrades type IV, V, VII, and X collagens. The protein degrades extracellular matrix proteins and acts on several non-matrix proteins such as big endothelial 1 (bET) and β-type calcitonin gene-related peptide (CGRP) promoting vasoconstriction. MMP-2 also cleaves KISS (metastasis suppressor) at a Gly-|-Leu bond. The protein appears to have a role in myocardial cell death pathways and contributes to myocardial oxidative stress by regulating the activity of glycogen synthase kinase-3 β (GSK3β). It has been shown to cleave GSK3β in vitro. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to fibroblast growth factor-2 (FGF2) and vitronectin.

Physikalische Form

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Rekonstituierung

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable