SRP6147

Ubiquitin Activating Enzyme E1 human

≥95% (SDS-PAGE)

• Synonym(e): AMCX1, POC20, UBA1, UBE1, Ubiquitin E1
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Assay: ≥95% (SDS-PAGE)
• Form: liquid
• Wirksamkeit: >3 nM
• Mol-Gew.: 118
• Verpackung: pkg of 50 μg
• NCBI-Hinterlegungsnummer: P22314
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... UBA1(7317)

Beschreibung

Allgemeine Beschreibung

Research area: Cell Signaling

Ubiquitin activating enzyme 1 (UBA1) or UBE1, is an ubiquitin-activating enzyme. The gene encoding it is localized on human chromosome Xp11.23.

Anwendung

Ubiquitin Activating Enzyme E1 human has been used in in vitro and in vivo ubiquitination assays.

Biochem./physiol. Wirkung

Ubiquitin activating enzyme 1 (UBA1) has a role in cellular protein homeostasis. It is the first enzyme utilized in the pathway to conjugate ubiquitin onto target substrates through an ubiquitin-protein isopeptide bond. The C-terminal glycine of an ubiquitin (G76) is initially adenylated in an ATP dependent manner activating it so it can be transferred to the active site cysteine of UBE1 forming an ubiquitin-UBE1 thiolester. With the help of the other enzymes in the ubiquitin conjugation pathway (E2/E3 enzymes) the ubiquitin is transferred to a specific lysine on a target substrate. The protein has been associated with spinal muscular atrophy (SMA) and Huntington′s disease (HD).

Physikalische Form

In 50 mM Tris (pH 8.0), 100 mM NaCl and 10% glycerol.

Angaben zur Herstellung

Centrifuge the vial prior to opening.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable