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Merck

SAB3701171

Sigma-Aldrich

Anti-Mouse IgG1 (γ-chain specific)-Peroxidase antibody produced in rabbit

affinity isolated antibody, lyophilized powder

Synonym(e):

HRP

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

rabbit

Qualitätsniveau

Konjugat

peroxidase conjugate

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Form

lyophilized powder

Speziesreaktivität

mouse

Methode(n)

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable

Versandbedingung

wet ice

Lagertemp.

2-8°C

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. It consists of a γ heavy chain in the constant (C) region. The monomeric 150kDa structure of IgG constitutes two identical heavy chains and two identical light chains with molecular weight of 50kDa and 25kDa, respectively. The primary structure of this antibody also contains disulfide bonds involved in linking the two heavy chains, linking the heavy and light chains and resides inside the chains. Maternal IgG is the only antibody transported across the placenta to the fetus. It passively immunizes the infants. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4 with different heavy chains, named γ1, γ2, γ3, and γ4, respectively. IgG1 is induced due to antibody responses to membrane proteins and soluble protein antigens. It is the most abundant subclass of IgG.

Spezifität

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA against other Mouse or Human heavy chain isotypes. Cross reactivity with Mouse IgG2b and IgG3 is negligible (<0.5%). Cross reactivity with Mouse IgG2a and IgM is minimal.

Immunogen

Mouse IgG1 heavy chain

Anwendung

Anti-Mouse IgG1 (γ-chain specific)-Peroxidase antibody produced in rabbit has been used in immunohistochemistry and Western blotting.

Physikalische Eigenschaften

Antibody format: IgG

Physikalische Form

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Rekonstituierung

Reconstitute with 1.0 mL deionized water (or equivalent).

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Piktogramme

Exclamation mark

Signalwort

Warning

H-Sätze

P-Sätze

Gefahreneinstufungen

Skin Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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B1 cells constitute a specialized subset of B cells, best characterized in mice, which is abundant in body cavities, including the peritoneal cavity. Through natural and antigen-induced antibody production, B1 cells participate in the early defense against bacteria. The G
Samantha M Solon-Biet et al.
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