RAB0874

Porcine INS / Insulin ELISA Kit

• UNSPSC-Code: 41116158
• NACRES: NA.32

Eigenschaften

• Speziesreaktivität: porcine
• Verpackung: kit of 96 wells (12 strips x 8 wells)
• Methode(n): ELISA: suitable
• Aufnahme: sample type plasma; sample type cell culture supernatant(s); sample type serum
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 4 μIU/mL; standard curve range: 4.69 μIU/ml-300 μIU/ml
• Nachweisverfahren: colorimetric
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: Porcine ... INS(397415)

Beschreibung

Allgemeine Beschreibung

The antibody reacts specifically against insulin by RIA and immunocytochemistry. It exhibits cross-reactivity with human proinsulin. The antibody binds to insulin with an affinity constant of 8.8 × 10⁹ M^-1 in RIA. The insulin gene is mapped to human chromosome 11p15.5.
Human insulin is made up of 51 amino acids. It consists of two peptide chains, 21 amino acids make up A-chain and chain B contains 31 amino acids. The two chains are linked together by a disulfide bond, to form a classical insulin fold with three α helices, chain A having two and one in chain B.

Anwendung

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem./physiol. Wirkung

Abnormal insulin secretion or function, leads to diabetes mellitus, obesity and other carbohydrate metabolism disorders. Insulin is known to stimulate cell division. The biological effects of insulin are mediated by insulin receptor and it integrates major signaling pathways.

Sonstige Hinweise

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit-Komponenten auch einzeln erhältlich

• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABWASH4 20X Wash Buffer (Item B)

Sicherheitshinweise

• Piktogramme: GHS05
• Signalwort: Warning
• H-Sätze: H290
• P-Sätze: P234 - P390
• Gefahreneinstufungen: Met. Corr. 1
• Lagerklassenschlüssel: 8A - Combustible corrosive hazardous materials
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable