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Merck

PP0201

Sigma-Aldrich

Glycoprofile II, Enzymatic In-Solution N-Deglycosylation Kit

sufficient for 20 samples

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About This Item

UNSPSC-Code:
12352202
NACRES:
NA.26

Qualitätsniveau

Lagertemp.

2-8°C

Allgemeine Beschreibung

Contains sufficient reagents for a minimum of 20 reactions when the sample size is between one to two mg of a typical glycoprotein.

Anwendung

GlycoProfile II Enzymatic In-Solution N-Deglycosylation Kit was used to study the effects of glycosylation of the N-acetyl-D-galactosamine (GalNAc)-specific lectin found in Schizophyllum commune.
GlycoProfile II has been optimized to provide a convenient and reproducible method to remove N-linked glycans from glycoproteins and is compatible with subsequent MALDI-TOF mass spectrometric analysis without interference from any of the reaction components.
Glycoprofile II, Enzymatic In-Solution N-Deglycosylation Kit has been used in the deglycosylation of Schizophyllum commune lectin, Arabidopsis mannanase, human umbilical vein endothelial cells (HUVEC) protein lysate and organic solute transporter protein, Ostβ.

Rechtliche Hinweise

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

Nur Kit-Komponenten

Produkt-Nr.
Beschreibung

  • 10× Reaction Buffer 1 mL/vial

Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB

  • P7367PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE), for proteomics 50 USDB

  • R7884Ribonuclease B from bovine pancreas, BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE) .5 mgSDB

  • O9882Octyl β-D-glucopyranoside, BioXtra, ≥98% (GC) 100 mgSDB

  • M31482-Mercaptoethanol, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) .9 mLSDB

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

Zielorgane

Liver,Heart

Lagerklassenschlüssel

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials


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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Podjana Chumkhunthod et al.
Biochimica et biophysica acta, 1760(3), 326-332 (2006-03-02)
An N-acetyl-D-galactosamine (GalNAc)-specific lectin was purified from the edible mushroom, Schizophyllum commune, using affinity chromatography on a porcine stomach mucin (PSM)-Sepharose 4B column. Under reducing and non-reducing conditions, SDS-polyacrylamide gel electrophoresis gave a major band of 31.5 kDa. The Schizophyllum
Purification and characterization of an N-acetyl-D-galactosamine-specific lectin from the edible mushroom Schizophyllum commune
Chumkhunthod P, et al.
Biochim. Biophys. Acta Gen. Subj., 1760(3), 326-332 (2006)
Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown
Haarmann A, et al.
PLoS ONE, 5(10), e13568-e13568 (2010)
Elena Schwagerus et al.
Pflugers Archiv : European journal of physiology, 467(11), 2257-2273 (2015-02-14)
Using human airway epithelial cell lines (i.e. NCI-H441 and Calu-3) as well as human alveolar epithelial type I-like (ATI) cells in primary culture, we studied the contribution of the epithelial sodium channel δ-subunit (δ-ENaC) to transepithelial sodium transport in human
Enzymatic characterization of a glycoside hydrolase family 5 subfamily 7 (GH5\_7) mannanase from Arabidopsis thaliana
Wang Y, et al.
Planta, 239(3), 653-665 (2014)

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