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Merck

P9827

Sigma-Aldrich

Polycytidylsäure-Agarose

lyophilized powder

Synonym(e):

Poly(C)–Agarose Kaliumsalz

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About This Item

MDL-Nummer:
UNSPSC-Code:
41106500
NACRES:
NA.56

Form

lyophilized powder

Qualitätsniveau

Kennzeichnungsgrad

0.25-1.0 mg per mL

Matrix

cross-linked 4% beaded agarose

Matrixaktivierung

cyanogen bromide

Matrixanbindung

amino

Matrix-Spacer

1 atom

Quellen

1 g swells to ~5 mL

Lagertemp.

−20°C

Anwendung

Polycytidylic acid-agarose is used in protein chromatography, affinity chromatography and specialy resins. Polycytidylic acid has been used to study the preferential induction of apoptosis in the rainbow trout macrophage cell line. Polycytidylic acid has also been used to develop two analytical methods for human secretory-type ribonuclease.

Physikalische Form

Lyophilisiertes Pulver stabilisiert mit Lactose

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


Analysenzertifikate (COA)

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J Yang et al.
Plant physiology, 98(3), 1115-1120 (1992-03-01)
A polyadenylate-binding protein (PABP) was purified from cell-free extracts prepared from pea seedlings (Pisum sativum) by ammonium sulfate precipitation and Affi-Gel Blue and polyadenylate-Sepharose 4B affinity chromatography. The final preparation from polyadenylate-Sepharose 4B columns contained a single 70-kilodalton polypeptide with
Cristiana Valle et al.
Parasitology research, 96(4), 199-205 (2005-04-15)
We have cloned, sequenced and expressed a gene of Haemonchus contortus that encodes a protein (termed HcCYP) consisting of a cyclophilin domain and an RNA recognition motif (RRM). An antiserum raised against the recombinant protein showed that HcCYP was present
S J Dewitte-Orr et al.
Fish & shellfish immunology, 18(4), 279-295 (2004-11-25)
The rainbow trout macrophage cell line RTS11 was found to be considerably more sensitive than rainbow trout fibroblast (RTG-2) and Chinook salmon epithelial (CHSE-214) cell lines to killing by macromolecular synthesis inhibitors, actinomycin D (AMD) and cycloheximide (CHX), a synthetic
Preparation of sepharose-bound poly (rI:rC).
A F Wagner et al.
Biochemical and biophysical research communications, 45(1), 184-189 (1971-10-01)
D Nadano et al.
Analytical biochemistry, 212(1), 111-116 (1993-07-01)
Two analytical methods for human secretory-type ribonuclease, which are based on polycytidylic acid/ethidium bromide fluorescence, have been developed. The first is a method for measurement of secretory-type ribonuclease activity utilizing the radial diffusion of ribonuclease in a thin agarose gel

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