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Merck

P8563

Sigma-Aldrich

Polyuridylsäure-Agarose

lyophilized powder, matrix polyacrylhydrazido-agarose

Synonym(e):

Poly(U)–Agarose Kaliumsalz

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About This Item

MDL-Nummer:
UNSPSC-Code:
41106500
NACRES:
NA.56

Form

lyophilized powder

Qualitätsniveau

Kennzeichnungsgrad

0.1-1.0 mg per mL

Matrix

polyacrylhydrazido-agarose

Matrixanbindung

ribose moiety after periodate oxidation

Quellen

1 g swells to ~4 mL

Kapazität

≥0.2 mg/mL binding capacity (poly(A) (P 9403))

Lagertemp.

−20°C

Anwendung

Polyuridylic acid-agarose is used in protein chromatography, affinity chromatography and specialty resins. Polyuridylic acid has been used to study insulin receptors and to develop polyclonal antibodies against RNase L.

Physikalische Form

Lyophilisiertes Pulver stabilisiert mit Lactose

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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T Salehzada et al.
The Journal of biological chemistry, 266(9), 5808-5813 (1991-03-25)
RNase L activated by 2-5A (a series of 2'-5'-linked adenylic oligoribonucleotides) is a key enzyme of the interferon system. To study RNase L (endonuclease L) in intact cells independently of intracellular 2-5A and of its activity, we have developed polyclonal
J P Medema et al.
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, 7(4), 543-550 (1996-04-01)
We have used two approaches to identify possible substrates of the insulin receptor (IR) tyrosine kinase. First, we used a potent tyrosine phosphatase inhibitor, phenylarsine oxide (PAO), which is reported to be specific for the insulin-induced signal transduction route, to
Eulàlia Belloc et al.
Nature, 452(7190), 1017-1021 (2008-04-04)
In vertebrate oocytes, meiotic progression is driven by the sequential translational activation of maternal messenger RNAs stored in the cytoplasm. This activation is mainly induced by the cytoplasmic elongation of their poly(A) tails, which is mediated by the cytoplasmic polyadenylation
L P Gavrilova et al.
FEBS letters, 178(2), 283-287 (1984-12-10)
The stoichiometry of GTP hydrolysis during peptide elongation in the processes of codon-specific translation and misreading of polyuridylic acid was determined in a cell-free system in which all ribosomes were active in peptide synthesis. Ribosomes carrying oligophenylalanine presynthesized on poly(U)
N J Horscroft et al.
The Journal of general virology, 81(Pt 8), 1961-1965 (2000-07-19)
The bluetongue virus ssRNA-binding protein, NS2, is a phosphoprotein that forms viral inclusion bodies in infected cells. Recombinant NS2 was expressed in the baculovirus expression system and purified to homogeneity from insect cells. Purified NS2 bound nucleosides. Further investigation revealed

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