I2393

IL-1β rat

≥98% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(e): IL-1β, Interleukin-1β from rat, Lymphocyte activating factor
• MDL-Nummer: MFCD01094098
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• product name: Interleukin-1β from rat, IL-1β, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• Biologische Quelle: rat
• Qualitätsniveau: 100
• Rekombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE and HPLC)
• Form: lyophilized powder
• Wirksamkeit: 0.01-0.1 ng/mL ED₅₀/EC₅₀
• Mol-Gew.: 17.3 kDa
• Verpackung: pkg of 10 and 50 μg
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: <0.1 EU/μg
• UniProt-Hinterlegungsnummer: Q63264
• Lagertemp.: −20°C
• Angaben zum Gen: rat ... Il1b(24494)

Beschreibung

Allgemeine Beschreibung

Interleukin-1 (IL-1), originally known as Lymphocyte Activating Factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin-2.¹ IL-1 is primarily released from stimulated macrophages and monocytes, but is also released from several other cell types,² and is thought to play a key role in inflammatory and immune responses.³ Other synonyms for IL-1 include: Endogenous Pyrogen (EP), Mitogenic Protein (MP), Helper Peak-1 (HP-1), T Cell Replacing Factor III (TRF III or TRF_H), B cell Activating Factor (BAF) and B Cell Differentiation Factor (BDF).⁴ The two closely related agents, Interleukin-1α (IL-1α) and Interleukin-1β (IL-1β) bind to the same cell surface receptor, elicit nearly identical biological responses and yet share 25% homology in their amino acid sequence. Recombinant rat IL-1β is a 17.3 kDa protein containing 153 amino acid residues.

Physikalische Form

Lyophilized from a 0.2 μm filtered buffered solution.

Hinweis zur Analyse

The biological activity is measured in a cell proliferation assay using murine D10S cells.

Quellenangabe

1. Gery, I., et al., J. Exp. Med., 136, 128 (1972).
2. Oppenheim, J. J., et al., Immunol. Today, 7, 45 (1986).
3. Durum, S. K., et al., Annu. Rev. Immunol., 3, 263 (1985).
4. Aarden, L., et al., J. Immunol., 123, 2928 (1979).
5. Symons, J., et al., Lymphokines and Interferons, A Practical Approach, Clemens, M., et al., (eds.), IRL Press, Oxford (1987).

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)