RAB0278

Rat IL-1 β ELISA Kit

for cell and tissue lysates

• Synonym(e): Il-1 beta, Interleukin-1 beta
• UNSPSC-Code: 41116158
• NACRES: NA.32

Eigenschaften

• Speziesreaktivität: rat
• Verpackung: kit of 96 wells (12 strips x 8 wells)
• Methode(n): ELISA: suitable; capture ELISA: suitable
• Aufnahme: sample type cell lysate; sample type tissue lysate
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 80 pg/mL; standard curve range: 68.59-50000 pg/mL
• Nachweisverfahren: colorimetric
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: rat ... Il1b(24494)

Beschreibung

Allgemeine Beschreibung

The Rat IL-1 β ELSA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-1β cell lysate and tissue lysate.

Immunogen

Recombinant Rat IL-1 β

Anwendung

For research use only. Not for use in diagnostic procedures

Rat IL-1 Β ELISA Kit has been used to measure IL-1 Β levels .
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem./physiol. Wirkung

Pro-inflammatory cytokine IL-1 Β (IL1B), which is highly expressed on Β-adrenoreceptor in obese adipose tissues, is implicated in induction of uncoupling protein 1 (UCP1) expression via extracellular signal-regulated kinase (ERK) activation in adipocytes. Macrophage autophagy inhibits the production of inflammasome-dependent IL-1Β and thereby it plays a vital role in reducing acute toxin-induced liver injury and death. In rats, IL-1Β enhanced neurogenesis and plasticity of carotid bodies (CB) by triggering ERK1/2 activation. IL-1Β plays a vital role in alteration of glomerular hemodynamics via stimulating the production of prostaglandin E2 (PGE2) in the mesangial cells.

Sonstige Hinweise

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit-Komponenten auch einzeln erhältlich

• RABDIL6 ELISA 5X Sample Diluent Buffer (Item D2)
• RABELADE ELISA 5X Assay/Sample Diluent Buffer E (Item E2)
• RABLYSIS1 2X Cell Lysis Buffer (Item J)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Sicherheitshinweise

• Piktogramme: GHS05
• Signalwort: Warning
• H-Sätze: H290
• P-Sätze: P234 - P390
• Gefahreneinstufungen: Met. Corr. 1
• Lagerklassenschlüssel: 8A - Combustible corrosive hazardous materials