H6291

IL-1β human

HumanKine^®, recombinant, Xeno-free, >95% (SDS-PAGE), suitable for cell culture

• Synonym(e): IL-1β, Lymphocyte activating factor
• MDL-Nummer: MFCD00131399
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• product name: Interleukin-1β human, IL-1β, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture, endotoxin tested
• Biologische Quelle: human
• Qualitätsniveau: 100
• Rekombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Wirksamkeit: ≤0.05 ng/mL EC₅₀
• Qualität: endotoxin tested
• Mol-Gew.: dimer 18-25 kDa (glycosylated)
• Verpackung: pkg of 10 μg
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: <1 EU/μg
• UniProt-Hinterlegungsnummer: P01584
• Lagertemp.: −20°C
• Angaben zum Gen: human ... IL1B(3553)

Beschreibung

Biochem./physiol. Wirkung

Interleukin-1 (IL-1), originally known as Lymphocyte Activating Factor (LAF), activates T cell lymphocytes, which then proliferate and secrete interleukin-2 (IL-2). IL-1 is released primarily from stimulated macrophages and monocytes, but has also been shown to be released from several other types and is thought to play a key role in inflammatory and immune responses. Other synonyms for IL-1 include: endogenous pyrogen (EP), mitogenic protein (MP), Helper Peak-1 (HP-1), T Cell Replacing Factor III (TRF III or TRFM), B Cell Activating Factor (BAF) and B Cell Differentiation Factor (BDF) The two closely related agents Interleukin-1α (IL-1α) and Interleukin-1β (IL-1β) share 62% homology in amino acid sequence and elicit nearly identical biological responses. IL-1α and IL-1β are both approximately 17 kDa with some heterogeneity in the amount of glycosylation.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Angaben zur Herstellung

HumanKine Recombinant Human IL-1β, expressed in human HEK 293 cells, is a glycosylated monomer with a molecular mass of 18-25 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Hinweis zur Analyse

The activity is determined by the dose-dependent stimulation of the proliferation of mouse D10S cells.

Rechtliche Hinweise

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable