H7039

3-Hydroxy-3-methylglutaryl-CoA reductase human

solution

• Synonym(e): HMGCoA Reductase, HMGR
• EC-Nummer: 1.1.1.34 (BRENDA, IUBMB)
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Assay: ≥90% (SDS-PAGE)
• Qualitätsniveau: 200
• Form: solution
• Spezifische Aktivität: 2-8 units/mg protein
• Mol-Gew.: ~76 kDa (for the fusion protein)
• UniProt-Hinterlegungsnummer: P04035
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... HMGCR(3156)

Beschreibung

Anwendung

3-Hydroxy-3-methylglutaryl-CoA reductase human has been used:

• in anti-HMGCR enzyme linked immunosorbent assay (ELISA)
• as an antigen to measure anti-HMGCR antibodies by blot analysis
• in HMG-CoA reductase activity assays
• as an antigen to compare in-house antigen via sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)/western blot

Biochem./physiol. Wirkung

3-Hydroxy-3-methylglutaryl-CoA reductase/HMGCR serves as a rate limiting enzyme in mevalonate pathway. It also controls the oncogenic Hippo transducers TAZ/YAP. HMGCR also perform as a genetic modifier for Alzheimer′s disease (AD).

Hydroxy-3-methylglutaryl-CoA reductase catalyzes the committed step in cholesterol biosynthesis.

Physikalische Eigenschaften

Consists of the HMG CoA reductase catalytic domain fused to GST protein.

Einheitendefinition

One unit will convert 1.0 μmole of NADPH to NADP⁺ per minute at 37 °C.

Physikalische Form

Supplied as a solution containing 250 μg protein in 50 mM Tris pH 7.5, with 5 mM DTT, 1:200 Protease Inhibitor Cocktail (Catalog Number P8340) and 50% (w/v) glycerol.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable