G4155

ββ-Galactosidase aus E. coli

aqueous glycerol suspension, ≥500 units/mg protein (biuret)

• Synonym(e): β-D-Galactosid-Galactohydrolase, Lactase
• CAS-Nummer: 9031-11-2
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 3.2.1.23 (BRENDA, IUBMB)
• MDL number: MFCD00155434
• Specific activity: ≥500 units/mg protein (biuret)

Eigenschaften

• form: aqueous glycerol suspension
• Quality Segment: 200
• specific activity: ≥500 units/mg protein (biuret)
• mol wt: 465 kDa
• storage temp.: −20°C

Beschreibung

General description

Molekulargewicht des Tetramers: 465 kDa (Subunits je 116,3 kDa)

Application

β-Galactosidase is used in the enzymatic assays in the synthesis of imidazolo-pyrrolidinoses.

Biochem/physiol Actions

β-Galactosidase spaltet Lactose in ihre Monosaccharid-Komponenten Glucose und Galactose. Außerdem katalysiert sie die Transglycosylierung von Glucose zu Allolactose, dem Induktor von β-Galactosidase, in einem Rückführungsmechanismus.

Physical form

Suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, 0.5 mM mercaptoethanol. pH 7.4

Other Notes

Loss of enzymatic activity can occur if this enzyme is frozen. This enzyme is provided in a 50% glycerol solution and will not freeze if stored at -20° Celsius. It is not recommended that this enzyme preparation be stored in an ultracold freezer (-60 to -100° Celsius).

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1