A search of the website does not show a density for product G4155. For products supplied in small quantities, it is not common for the density to be measured. However, for solutions available in larger quantities, density information is often provided. Although the density for G4155 is not stated, there is some useful information available from the website. The physical form of the product is a suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, and 0.5 mM mercaptoethanol at pH 7.4.
Fortfahren mit
G4155
ββ-Galactosidase aus E. coli
aqueous glycerol suspension, ≥500 units/mg protein (biuret)
Synonym(e):
β-D-Galactosid-Galactohydrolase, Lactase
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Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥500 units/mg protein (biuret)
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltenform
aqueous glycerol suspension
Quality Segment
specific activity
≥500 units/mg protein (biuret)
mol wt
465 kDa
storage temp.
−20°C
General description
Molekulargewicht des Tetramers: 465 kDa (Subunits je 116,3 kDa)
Application
β-Galactosidase is used in the enzymatic assays in the synthesis of imidazolo-pyrrolidinoses.
Biochem/physiol Actions
β-Galactosidase spaltet Lactose in ihre Monosaccharid-Komponenten Glucose und Galactose. Außerdem katalysiert sie die Transglycosylierung von Glucose zu Allolactose, dem Induktor von β-Galactosidase, in einem Rückführungsmechanismus.
Physical form
Suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, 0.5 mM mercaptoethanol. pH 7.4
Other Notes
Loss of enzymatic activity can occur if this enzyme is frozen. This enzyme is provided in a 50% glycerol solution and will not freeze if stored at -20° Celsius. It is not recommended that this enzyme preparation be stored in an ultracold freezer (-60 to -100° Celsius).
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| specific activity ≥500 units/mg protein (biuret) | specific activity ~140 U/mg | specific activity ≥500 units/mg protein | specific activity ≥500 units/mg protein |
| form aqueous glycerol suspension | form powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 465 kDa | mol wt Mr ~135000 | mol wt 465 kDa | mol wt 465 kDa |
| Quality Level 200 | Quality Level 100 | Quality Level 300 | Quality Level 200 |
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Lagerklasse
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
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Verwandter Inhalt
QC Methods
Global Trade Item Number
| SKU | GTIN |
|---|---|
| G4155-3KU | 04061833630501 |
| G4155-1KU | 04061833630495 |
| G4155-5KU | 04061832843810 |
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Could you please advise if the density of the aqueous suspension of beta galactosidase from Escherichia coli (G4155) can be assumed as 1 uL of solution is equal to 1 mg, given that the SDS and provided enzyme assay do not contain this information?
1 answer-
Helpful?
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We recently purchased this enzyme. Information given regarding enzyme volume is only 1000 units. I want to know what would be units per uL. How much is the liquid volume in the given product to calculate the enzyme concentration? Provide proper informatio
1 answer-
Units/ml can be calculated using the numbers reported in batch specific CofA. Below is an example.
CofA of G4155, lot 0000303635
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/G4155-3KU0000303635.pdf
1.5 mgP/ml X 766 units/mg Protein = 1149 units/mlHelpful?
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