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E4643

Sigma-Aldrich

Heparin-Binding EGF-Like Growth Factor human

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

Synonym(e):

hHB-EGF, HB-EGF, Heparin-Binding Epidermal Growth Factor-Like Growth Factor

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About This Item

MDL-Nummer:
MFCD00212935
UNSPSC-Code:
12352202
NACRES:
NA.77

product name

Heparin-Binding EGF-Like Growth Factor human, HB-EGF, recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

Biologische Quelle

human

Qualitätsniveau

100

Rekombinant

expressed in baculovirus infected Sf21 cells

Assay

≥97% (SDS-PAGE and N-terminal analysis)

Form

lyophilized powder

Wirksamkeit

≤3 ng/mL

Qualität

endotoxin tested

Mol-Gew.

9.5 kDa

Verpackung

pkg of 50 and 250 μg

Lagerbedingungen

avoid repeated freeze/thaw cycles

Methode(n)

cell culture | mammalian: suitable

UniProt-Hinterlegungsnummer

Q99075

Lagertemp.

−20°C

Angaben zum Gen

human ... HBEGF(1839) , HBEGF(1839)

Biochem./physiol. Wirkung

Heparin-binding epidermal growth factor (HB-EGF), like BTC, is a heparin-binding EGF family member. Natural soluble HB-EGF is a 19 kDa glycoprotein of multiple forms due to heterogeneous truncations. Soluble HB-EGF is mitogenic and chemotactic for fibroblasts, keratinocytes, and smooth muscle cells but not capillary endothelial cells. Production of HB-EGF is detected in monocytes, macrophages, and vascular and aortic smooth muscle cells (SMC). HB-EGF may play a role in the pathogenesis of atherosclerosis. It may also be an effector of Jun-induced oncogenic transformation. HB-EGF binds to EGFR with high affinity and to ErbB with moderate affinity.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Hinweis zur Analyse

The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in the EGF-responsive BALB/3T3 cells.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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F Vinante et al.
Blood, 94(9), 3169-3177 (1999-11-11)
Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a widely expressed EGF superfamily member that induces mitogenic and/or chemotactic activities toward different cell types through binding to EGF receptors 1 or 4. Membrane-bound HB-EGF exerts growth activity and adhesion capabilities
M Lanzrein et al.
The Biochemical journal, 310 ( Pt 1), 285-289 (1995-08-15)
Preincubation of Vero cells with 1 microM phorbol 12-myristate 13-acetate (PMA) decreased the specific binding of diphtheria toxin by about 50%, whereas the toxic effect, endocytic uptake and membrane translocation were completely blocked. Toxin bound to PMA-treated cells was released
R Iwamoto et al.
The Journal of biological chemistry, 274(36), 25906-25912 (1999-08-28)
Heparin-binding epidermal growth factor-like growth factor (HB-EGF) transduces mitogenic signals through the EGF receptor (EGFR). There are two forms of HB-EGF, the membrane-anchored form (pro-HB-EGF) and the soluble form (sHB-EGF). We studied the biological activity of pro-HB-EGF by using a
U Palmer et al.
The European respiratory journal, 14(2), 405-411 (1999-10-09)
Signalling through epidermal growth factor (EGF) receptor leads to several cellular responses including cell division and cell migration. Since EGF receptors are expressed on normal mesothelial cells, this study investigated whether EGF receptor ligands act as chemoattractants on these cells.
S l Fu et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(10), 5716-5721 (1999-05-13)
Jun is a transcription factor belonging to the activator protein 1 family. A mutated version of Jun (v-Jun) transduced by the avian retrovirus ASV17 induces oncogenic transformation in avian cell cultures and sarcomas in young galliform birds. The oncogenicity of
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