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C7762
αα-Chymotrypsin aus Rinderpankreas
Type I-S, essentially salt-free, lyophilized powder
Synonym(e):
α-chymotrypsin A and B, alpha-chymotrypsin
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About This Item
Empfohlene Produkte
Typ
Type I-S
Qualitätsniveau
Form
essentially salt-free, lyophilized powder
Spezifische Aktivität
≥40 units/mg protein
Mol-Gew.
25 kDa
Aufgereinigt durch
3× crystallization
Löslichkeit
1 mM HCl: soluble 2.0 mg/mL, clear
UniProt-Hinterlegungsnummer
Lagertemp.
−20°C
Angaben zum Gen
cow ... CTRB1(618826)
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Anwendung
α-Chymotrypsin from bovine pancreas has been used in a study to investigate protein extraction by Winsor-III microemulsion systems. α-Chymotrypsin from bovine pancreas has also been used in a study to investigate a new specific fullerene-based fluorescent probe for trypsin.
The product has been used to investigate the inhibitory effect of several ether oligomers against the enzyme. It has also been used to cleave pro-phenoloxidase in order to estimate total phenoloxidase in haemolymph. Furthermore, the enzyme has been used as a positive control in chymotrypsin assays using salivary gland and anterior midgut extracts of Deraeocoris nigritulus.
Biochem./physiol. Wirkung
α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, as well as 10 mM of Cu2+ and Hg2+.
Ein Serin-Protease, die Peptidbindungen mit aromatischen oder großen hydrophoben Seitenketten (Tyr, Trp, Phe, Met, Leu) am Carboxylende der Bindung hydrolysiert.
Einheitendefinition
Eine Einheit hydrolysiert 1.0 μmol BTEE pro min bei pH 7.8 und 25 °C.
Angaben zur Herstellung
Prepared free of autolysis products and low molecular weight contaminants.
The powder may be reconstituted in 1 mM HCl at 2 mg/mL concentration to form a clear solution.
Hinweis zur Analyse
Minimum 85% protein
Protein determined by E1%/280
Sonstige Hinweise
Finden Sie mehr Informationen zu Chymotrypsin auf unserer 'Enzyme-Explorer'-Webseite.
Inhibitor
Produkt-Nr.
Beschreibung
Preisangaben
Substrat
Produkt-Nr.
Beschreibung
Preisangaben
Signalwort
Danger
Gefahreneinstufungen
Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Zielorgane
Respiratory system
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 1
Persönliche Schutzausrüstung
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Olivier Renaudet et al.
Organic letters, 6(3), 397-400 (2004-01-30)
[structure: see text] Hydroxyaromatic aldehydes and ketones were used as building blocks to prepare ether oligomers. An iterative two-step protocol involving Mitsunobu coupling and carbonyl reduction provided a protecting-group-free route with high yields. Activity screening of an 84-member library against
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Solution scattering is a powerful means of determining the overall arrangement of domains in the multidomain proteins of complement. the serine-proteinase domain is central to all proteolytic events during complement activation. As models of this domain, bovine beta-trypsin, trypsinogen, alpha-chymotrypsin
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