A7189

L-Alanin-Dehydrogenase aus Bacillus subtilis

ammonium sulfate suspension, ≥20 units/mg protein (Lowry)

• Synonym(e): L-Alanin: NAD⁺ -Oxidoreduktase
• CAS-Nummer: 9029-06-5
• EC-Nummer: 1.4.1.1 (BRENDA, IUBMB)
• EG-Nummer: 232-847-9
• MDL-Nummer: MFCD00131441
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: Bacillus subtilis
• Qualitätsniveau: 200
• Form: ammonium sulfate suspension
• Spezifische Aktivität: ≥20 units/mg protein (Lowry)
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

L-Alanine Dehydrogenase has a N-terminal substrate-binding domain and a C-terminal NAD-binding domain.

Anwendung

L-Alanine Dehydrogenase from Bacillus subtilis has been used in the carbon nanotube columns for H2-driven biocatalysis hydrogenation studies.

L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection .

Biochem./physiol. Wirkung

L-Alanine Dehydrogenase is essential for sporulation in Bacillus subtilis.

L-Alanine dehydrogenase is a stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate and NADH are released in that specific order. Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine.

Einheitendefinition

One unit will convert 1.0 μmole of L-alanine to pyruvate and NH₃ per min at pH 10.0 at 25 °C.

Physikalische Form

Suspension in 2.4 M (NH₄)₂SO₄ solution, pH 7.0

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)