SAE0023

Alanine Racemase from Bacillus stearothermophilus

buffered aqueous solution, ≥10 IU/mg solid

• Synonym(e): Alanine Racemase from Bacillus stearothermophilus
• CAS-Nummer: 9024-06-0
• EC-Nummer: 5.1.1.1 (BRENDA, IUBMB)
• MDL-Nummer: MFCD03454300
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: Bacillus sp. (B. stearothermophilus)
• Qualitätsniveau: 200
• Form: buffered aqueous solution
• Spezifische Aktivität: ≥10 IU/mg solid
• Mol-Gew.: M_r 78 kDa (2 subunits 39 kDa each)
• Lagertemp.: −20°C

Beschreibung

Anwendung

Alanine racemase is used to convert L-alanine into D-alanine. Alanine racemase, from Sigma, has been used to isomerize L-[U-¹⁴C]alanine to a racemic mixture of L/D- [¹⁴C]alanine †.

Biochem./physiol. Wirkung

Alanine racemase is involved in alanine, aspartate and D-alanine metabolism. 3-Fluoro-D-alanine and D-Cycloserine are known to inhibit alanine racemase.† Alanine racemase monomer is composed of two domains, an eight-stranded α/β barrel at the N-terminus and a C-terminal domain. The N-terminus includes residues 1-240, whereas the C-terminal comprises of the β-strand (residues 241-388). One molecule of pyridoxalphosphate (PLP) is present as the cofactor in each subunit.†

Einheitendefinition

One unit will convert 1.0 μmole of D-alanine to L-alanine per minute at pH 10.5 at 30 °C in a coupled assay system with L-alanine dehydrogenase.

Physikalische Form

Aqueous solution containing 10 mM potassium phosphate

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable