A2901
Avidin–FITC from egg white
lyophilized powder
Synonym(e):
Avidin–Fluorescein isothiocyanate from egg white
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About This Item
Empfohlene Produkte
Konjugat
FITC conjugate
Qualitätsniveau
Form
lyophilized powder
Kennzeichnungsgrad
2-4 mol fluorescein isothiocyanate per mol avidin
Methode(n)
immunohistochemistry: suitable
Lagertemp.
−20°C
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Allgemeine Beschreibung
Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.
Anwendung
Avidin-FITC from egg white has been used for immunohistochemistry applications.
To determine the amount of labeled polyclonal IgG present in spores in an IgG prep. A known concentration of FITC avidin from egg white and biotin-IgG avidin was used in excess at 300nm.
Verpackung
Package size based on protein content
Physikalische Form
Contains sodium citrate
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Resp. Sens. 1 - Skin Sens. 1
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 29(4), 577-580 (1981-04-01)
The use of avidin-biotin interaction in immunoenzymatic techniques provides a simple and sensitive method to localize antigens in formalin-fixed tissues. Among the several staining procedures available, the ABC method, which involves an application of biotin-labeled secondary antibody followed by the
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