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Wichtige Dokumente
11207750910
Roche
Anti-Digoxigenin-Rhodamine, Fab fragments
from sheep
Synonym(e):
anti-digoxigenin, digoxigenin
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
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About This Item
UNSPSC-Code:
12352203
Empfohlene Produkte
Biologische Quelle
sheep
Qualitätsniveau
Konjugat
rhodamine conjugate
Antikörperform
purified antibody
Antikörper-Produkttyp
primary antibodies
Klon
polyclonal
Form
lyophilized (clear, red solution after reconstitution)
Verpackung
pkg of 200 μg
Hersteller/Markenname
Roche
Isotyp
IgG
Lagertemp.
2-8°C
Allgemeine Beschreibung
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to rhodamine.
Spezifität
Polyclonal antibodies from sheep show 100% reactivity with digoxigenin and digoxin, but no cross-reactivity with other steroids, such as human estrogens (e.g., estradiol) or androgens (e.g., testosterone).
Anwendung
Use Anti-Digoxigenin-Rhodamine, Fab fragments for the detection of digoxigenin-labeled compounds using:
- Digoxigenin-labeled sugars in glycoconjugate research
- Fluorescent in situ hybridization (FISH)
- Immunohistocytochemistry
- In situ hybridization
- DNA fiber assay
Angaben zur Herstellung
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.
Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: Detection of digoxigenin-labeled sugars in glycoconjugates: 20 to 50 μg/ml
Working solution: PBS, 0.5% bovine serum albumin (w/v), pH 7.4.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. Furthermore pH can be increased up to pH 8.5 to 9.0.
Storage conditions (working solution): Always prepare fresh!
Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: Detection of digoxigenin-labeled sugars in glycoconjugates: 20 to 50 μg/ml
- Fluorescent in situ hybridization (FISH): 1 to 20 μg/ml
- Immunohistocytochemistry: 20 to 50 μg/ml
- In situ hybridization: 20 to 50 μg/ml
Working solution: PBS, 0.5% bovine serum albumin (w/v), pH 7.4.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. Furthermore pH can be increased up to pH 8.5 to 9.0.
Storage conditions (working solution): Always prepare fresh!
Rekonstituierung
Add 1 ml double-distilled water to a final concentration of 200 μg/ml.
Sonstige Hinweise
For life science research only. Not for use in diagnostic procedures.
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Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Aquatic Chronic 3 - Skin Sens. 1
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 2
Flammpunkt (°F)
does not flash
Flammpunkt (°C)
does not flash
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