84655
Schiff′s Reagens auf Aldehyd
for microscopy
Synonym(e):
Fuchsin sulfurous acid
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About This Item
UNSPSC-Code:
12352300
NACRES:
NA.21
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Allgemeine Beschreibung
Schiff′s reagent is used for the qualitative or quantitative assay of carbohydrates. Periodic acid-Schiff′s reagent (PAS) stain is broadly applicable in histology for visualizing glycogen, glycoproteins, mucins, and other polysaccharides in tissue and cells.
Anwendung
Schiff′s reagent for aldehydes has been used:
- in microtiter PAS assay to quantify the amount of secreted mucin in Vibrio cholera infected water
- to stain the colonies of Clostridium perfringens in PGY agar plates to identify plasmalogen-deficient mutants
- in periodic acid-Schiff′s reagent assay to quantify carbohydrates in a microtiter plate format
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Carc. 1B - Met. Corr. 1
Lagerklassenschlüssel
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves
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David R Jackson et al.
ACS chemical biology, 16(1), 6-13 (2020-12-23)
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Michelle Kilcoyne et al.
Analytical biochemistry, 416(1), 18-26 (2011-05-31)
Microtiter plate colorimetric assays are widely used for analysis of carbohydrates and glycoconjugates. However, mucins are often not easily detected, as they have low neutral sugar content. We have adapted and optimised the periodic acid-Schiff's reagent (PAS) staining for microtiter
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