80635

PIPES

BioXtra, for molecular biology, ≥99.5% (T)

• Synonym(e): 1,4-Piperazindiethansulfonsäure, Piperazin-1,4-bis(2-ethansulfonsäure), Piperazin-N,N′-bis-(2-ethansulfonsäure)
• Empirische Formel (Hill-System): C₈H₁₈N₂O₆S₂
• CAS-Nummer: 5625-37-6
• Molekulargewicht: 302.37
• Beilstein: 817713
• EG-Nummer: 227-057-6
• MDL-Nummer: MFCD00006159
• UNSPSC-Code: 12161700
• PubChem Substanz-ID: 57652837
• NACRES: NA.25

Eigenschaften

• Qualität: for molecular biology
• Qualitätsniveau: 100
• Produktlinie: BioXtra
• Assay: ≥99.5% (T)
• Form: powder
• Verunreinigungen: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• Glührückstand: ≤0.2% (as SO₄)
• Verlust: ≤0.2% loss on drying, 110 °C
• Nützlicher pH-Bereich: 6.1-7.5
• pKa (25 °C): 6.8
• mp (Schmelzpunkt): >300 °C (lit.)
• Löslichkeit: 0.1 M NaOH: 0.1 M at 20 °C, clear, colorless
• Anionenspuren: chloride (Cl^-): ≤200 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• Kationenspuren: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤100 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 0.1 M in 1 M NaOH
• UV-Absorption: λ: 260 nm A_max: 0.055; λ: 280 nm A_max: 0.040
• SMILES String: OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)
• InChIKey: IHPYMWDTONKSCO-UHFFFAOYSA-N

Beschreibung

Anwendung

• Sensitive Detection of Immunoglobulin G Stability Using in Real-Time Isothermal Differential Scanning Fluorimetry: Determinants of Protein Stability for Antibody-Based Therapeutics.: The use of PIPES buffer in this study was integral to maintaining the stability of immunoglobulin G during differential scanning fluorimetry. This method is crucial for evaluating the stability of antibody-based therapeutics (Moggridge et al., 2017).

Sonstige Hinweise

Hybrid selection of mRNA - component of prehybridization buffer

Sicherheitshinweise

• Lagerklassenschlüssel: 13 - Non Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)