71196

Natriumacetat -Lösung

BioUltra, for molecular biology, ~3 M in H₂O

• Empirische Formel (Hill-System): C₂H₃NaO₂
• CAS-Nummer: 127-09-3
• Molekulargewicht: 82.03
• Beilstein: 3595639
• MDL-Nummer: MFCD00012459
• UNSPSC-Code: 12161700
• eCl@ss: 39021906
• PubChem Substanz-ID: 57652229
• NACRES: NA.21

Eigenschaften

• Qualität: for molecular biology
• Qualitätsniveau: 200
• Produktlinie: BioUltra
• Form: liquid
• Konzentration: ~3 M in H₂O
• Methode(n): precipitation assay: suitable
• Verunreinigungen: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pH-Wert: 7.0-8.0 (25 °C, 3 M in H₂O)
• Anionenspuren: chloride (Cl^-): ≤10 mg/kg; phosphate (PO₄^3-): ≤5 mg/kg; sulfate (SO₄^2-): ≤20 mg/kg
• Kationenspuren: Al: ≤1 mg/kg; As: ≤0.1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤50 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• λ: 3 M in H₂O
• UV-Absorption: λ: 260 nm A_max: ≤0.030; λ: 280 nm A_max: ≤0.024
• SMILES String: [Na+].CC([O-])=O
• InChI: 1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1
• InChIKey: VMHLLURERBWHNL-UHFFFAOYSA-M

Beschreibung

Anwendung

Sodium acetate solution has been used:

• in DNA extraction
• in reverse crosslinking and DNA purification
• to precipitate Gata6 (GATA binding protein 6) cDNA as a part of high-purity plasmid preparation

Biochem./physiol. Wirkung

Sodium acetate serves as a fixed base. It is useful to rectify acidosis among acidotic patients. Orginally, sodium acetate is used as dialysate for hemodialysis, but is known to cause cardiovascular instability.

Sonstige Hinweise

Basic method of DNA precipitation with ethanol

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves