MABN1188

Anti-Tyrosine Hydroxylase Antibody

mouse monoclonal, 10F8.2

• Synonym(e): TH, Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, Tyrosine Hydroxylase
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• product name: Anti-Tyrosine Hydroxylase Antibody, clone 10F8.2, clone 10F8.2, from mouse
• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 10F8.2, monoclonal
• Speziesreaktivität: human, rat
• Speziesreaktivität (Voraussage durch Homologie): mouse (based on 100% sequence homology)
• Methode(n): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotyp: IgG2bλ
• NCBI-Hinterlegungsnummer: NP_036872
• UniProt-Hinterlegungsnummer: P04177
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... TH (7054)

Beschreibung

Allgemeine Beschreibung

Tyrosine 3-monooxygenase (EC 1.14.16.2; UniProt P04177; also known as TH, Tyrosine 3-hydroxylase) is encoded by the Th gene (Gene ID 25085) in rat species. Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines, dopamine and norepinephrine, from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. TH enzymatic activity requires ferrous ions as cofactor and is believed to be regulated by phosphorylation. Six human TH isoforms exist as a result of alternative splicing (UniProt P07101-1 through P07101-6).

Spezifität

Clone 10F8.2 targets a C-terminal epitope present in rat and mouse TH sequence (UniProt P04177 & P24529), as well as in all six human TH spliced isoforms (UniProt P07101).

Immunogen

Epitope: C-terminal region.

KLH-conjugated linear peptide corresponding to a C-terminal region sequence of rat tyrosine hydroxylase.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Neuronen- & Gliamarker

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected tyrosine hydroxylase in rat brain tissue sections.

This Anti-Tyrosine Hydroxylase Antibody, clone 10F8.2 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Tyrosine Hydroxylase.

Qualität

Evaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected tyrosine hydroxylase in 10 µg of PC12 cell lysate.

Zielbeschreibung

~50-60 kDa observed. 55.83 kDa (rat), 55.86 kDa (mouse), 58.03/55.48/58.47/55.92/45.21/44.77 kDa (human isoform 1/2/3/4/5/6) calculated (Met1 removed). The multiplet banding pattern observed in rat PC12 lysate most likely represent differentially phosphorylated TH species (Gozal, E., et al. (2005). J. Appl. Physiol. 99(2):642-649).

Verlinkung

Replaces: MAB318

Physikalische Form

Protein G purified.

Format: Purified

Purified mouse monoclonal IgG2bλ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable