MAB88916

Anti-Fibronectin Antibody

CHEMICON^®, mouse monoclonal, 3E3

• Synonym(e): Anti-CIG, Anti-ED-B, Anti-FINC, Anti-FN, Anti-FNZ, Anti-GFND, Anti-GFND2, Anti-LETS, Anti-MSF, Anti-SMDCF
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Produktbezeichnung: Anti-Fibronectin Frag Antibody, cell attachment fragment, clone 3E3, clone 3E3, Chemicon^®, from mouse
• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 3E3, monoclonal
• Speziesreaktivität: human
• Hersteller/Markenname: Chemicon^®
• Methode(n): ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG1
• NCBI-Hinterlegungsnummer: NM_002026.2; NM_054034.2; NM_212474.1; NM_212475.1; NM_212476.1; NM_212478.1; NM_212482.1
• UniProt-Hinterlegungsnummer: P02751
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... FN1(2335)

Beschreibung

Spezifität

The antibody reacts with human fibronectin and with a peptic fragment (11.5 kD) from human fibronectin (Pierschbacker et al., 1981) comprimising the cell attachment site (Pierschbacker et al., 1981; Pierschabacker et al., 1983, 1984; Hayman et al., 1985; Ruoslahti et al., 1986; Hynes, 1985). It inhibits the attachment of normal rat kidney cells to human fironectin coated plastic surface (Pierschbacker et al., 1981). Binding of the antibody to fibronectin cannot be inhibited by cell adhesion peptides from fibronectin having the sequence Arg-Gly-Asp of Gly-Arg-Gly-Asp-Ser (Pierschabacker et al., 1984; Hayman et al., 1985; Ruoslahti et al., 1986).

Immunogen

Epitope: cell attachment fragment

Purified human-fibronectin from amnion liquid.

Anwendung

Research Category
Zellstruktur

Research Sub Category
ECM-Proteine

Anti-Fibronectin Frag Antibody, cell attachment fragment, clone 3E3 is an antibody against Fibronectin Frag for use in ELISA, WB, IC, IH.

Immunocytochemistry: (5-20 μg/mL) Immunohistochemistry: (5-20 μg/mL) (Not tested on paraffin sections) Immunoblotting: (5-20 μg-mL)

ELISA

Inactivation or inhibition studies in cell culture.

Optimal working dilutions must be determined by end user.

Physikalische Form

Format: Purified

Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Lagerung und Haltbarkeit

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable