MAB3806

Anti-ATM phosphoSer1981 Antibody, clone 10H11.E12

clone 10H11.E12, Chemicon^®, from mouse

• Synonym(e): Ataxia Telangiectasia Mutated
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 10H11.E12, monoclonal
• Speziesreaktivität: mouse, human
• Hersteller/Markenname: Chemicon^®
• Methode(n): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NM_000051.3; NM_138292.3
• UniProt-Hinterlegungsnummer: Q13315
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: phosphorylation (pSer1981)
• Angaben zum Gen: human ... ATM(472)

Beschreibung

Allgemeine Beschreibung

ATM is a 370 kDa nuclear phosphoprotein involved in the autosomal recessive disease Ataxia Telangiectasia (AT). Ataxia telangiectasia (AT) is a debilitating neurodegenerative disease that occurs early in childhood, resulting in ataxic movements and speech defects caused by cerebellar degeneration. The underlying cause of the disease is a biochemical dysfunction in the cellular response to specific types of DNA damage, correlated with mutations in the protein kinase ATM (Ataxia telangiectasia mutated). ATM not only is key in the signaling cascade that responds to DNA double-stranded breaks, but it also controls cell-cycle checkpoints and is therefore important in cancer prevention.

Spezifität

Reacts with ATM Kinase phosphorylated at serine 1981. Clone 10H11.E12 is covered by US patent No. 6,916,627 and 7,108,992.

Immunogen

Epitope: phosphoSer1981

Synthetic peptide corresponding to amino acids 1974-1988 of human ATM (SLAFEEG[pS]QSTTISS).

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Zellzyklus, DNA-Replikation & -Reparatur

Anti-ATM Antibody, phosphoSer1981, clone 10H11.E12 is a Mouse Monoclonal Antibody for detection of ATM also known as Ataxia Telangiectasia Mutated & has been validated in IP, WB, ICC.

Western blot: Detects a 370 kDa protein in crude extracts from irradiated human foreskin or mouse 3T3L1 cells.

Immunocytochemistry: Foci are detected in irradiated human and mouse fibroblasts.

Immunoprecipitation: The antibody immunoprecipitates ATM from irradiated human and mouse cells.

Optimal working dilutions must be determined by end user.

Physikalische Form

Format: Purified

Purified immunoglobulin presented as a liquid in 0.02M phosphate buffer containing 0.25M NaCl and 0.1% sodium azide.

Lagerung und Haltbarkeit

Maintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.



This antibody and certain aspects of its use are disclosed and claimed in pending U.S. Patent Applications published as U.S. Patent Publication Nos. 2003/0077661 and 2003/0157572.

Hinweis zur Analyse

Control
Irradiated normal Human fibroblasts (no reactivity against non-irradiated cell extracts)

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable