MAB2253

Anti-Integrin β1 Antibody, clone 6S6

clone 6S6, Chemicon^®, from mouse

• Synonym(e): CD29
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 6S6, monoclonal
• Speziesreaktivität: human
• Hersteller/Markenname: Chemicon^®
• Methode(n): flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• Isotyp: IgG1
• NCBI-Hinterlegungsnummer: NM_002292.3
• UniProt-Hinterlegungsnummer: P05556
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... ITGB1(3688)

Beschreibung

Allgemeine Beschreibung

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.

Spezifität

Reacts with the human integrin beta1 subunit. Specificity verified by preclearing of beta1in immunoprecipitation, flow cytometry on transfectant cells displaying human beta1 integrin, and reactivity with purified beta1. Similar in behavior and characteristics to antibody clone 3S3 (Wilkins et al., 1996).

Immunogen

Human synovial cells

Anwendung

Research Category
Zellstruktur

Research Sub Category
Integrine

Detect Integrin β1 using this Anti-Integrin β1 Antibody, clone 6S6 validated for use in FC, IH & IP.

Immunoprecipitation

Immunohistochemistry on frozen sections

Flow cytometry

Blocks adhesion of cells to extracellular matrix proteins

Induces aggregation of certain lymphoid cells

Does not bind reduced beta1 integrin on Western blot

Optimal working dilutions must be determined by end user.

Zielbeschreibung

88 kDa

Physikalische Form

Protein A purified

Format: Purified

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Lagerung und Haltbarkeit

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hinweis zur Analyse

Control
Tonsil, human skin tissue

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable