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MAK165

Sigma-Aldrich

Fluorimetric Hydrogen Peroxide Assay Kit

sufficient for 500 fluorometric tests (red fluorescence)

Synonym(s):

Hydrogen Peroxide Quantification Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 500 fluorometric tests (red fluorescence)

detection method

fluorometric

relevant disease(s)

cardiovascular diseases; aging/geriatric diseases; orthopedic diseases; pulmonary disorders; neurological disorders

storage temp.

−20°C

Related Categories

General description

Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

This kit is suitable to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions.

Principle

The Fluorescent Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions. This kit can also be used to detect hydrogen peroxide released from living cells or produced by oxidase activities. This kit utilizes a peroxidase substrate that generates a red fluorescent product (λex = 540/λem = 590 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H302,H334

Hazard Classifications

Acute Tox. 4 Oral - Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
3510842
3320382
3320382
3510842
304139

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Wei Zhu et al.
Oxidative medicine and cellular longevity, 2019, 5305014-5305014 (2019-06-11)
Females develop kidney stones less frequently than males do. However, it is unclear if this gender difference is related to altered estrogen/estrogen receptor (ER) signaling. Here, we found that ER beta (ERβ) signals could suppress hepatic oxalate biosynthesis via transcriptional
Yue Yang et al.
The Journal of biological chemistry, 294(23), 9295-9307 (2019-04-06)
Interest in pharmacological agents capable of increasing cellular NAD+ concentrations has stimulated investigations of nicotinamide riboside (NR) and nicotinamide mononucleotide (NMN). NR and NMN require large dosages for effect. Herein, we describe synthesis of dihydronicotinamide riboside (NRH) and the discovery
Foteini Vasilopoulou et al.
GeroScience, 43(2), 965-983 (2020-11-01)
Brain aging and dementia are current problems that must be solved. The levels of imidazoline 2 receptors (I2-IRs) are increased in the brain in Alzheimer's disease (AD) and other neurodegenerative diseases. We tested the action of the specific and selective
Yan Zhang et al.
Journal of nanobiotechnology, 19(1), 161-161 (2021-06-02)
As one typical cardiovascular disease, atherosclerosis severely endanger people' life and cause burden to people health and mentality. It has been extensively accepted that oxidative stress and inflammation closely correlate with the evolution of atherosclerotic plaques, and they directly participate
Ellen E Salmon et al.
Cells, 9(5) (2020-05-28)
Clinical studies have established a connection between oxidative stress, aging, and atherogenesis. These factors contribute to senescence and inflammation in the endothelium and significant reductions in endothelium-dependent vasoreactivity in aged patients. Tissue-engineered blood vessels (TEBVs) recapitulate the structure and function
View All Related Papers

Questions

1–7 of 7 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  3. Can we use Assay buffer MAK165C included in the kit to lyse cells to make cellular extract to measure endogenous H2O2 level.

    1 answer

    1. Yes, the Assay buffer that comes as one of the kit components (Product MAK165C) can be used to lyse cells. The kit provides 100 mL of Assay buffer, which is more than enough for the assays as well as to use some for sample preparation. MAK165C does not contain a surfactant, so the cells would need to be lysed by physical disruption...either sonication, or using a Dounce homogenizer (or equivalent).

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  4. What is the limit of detection for H2O2?

    1 answer

    1. The lower limit of detection for kit MAK165 is 0.06 uM.

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  5. Does MAK165 recognize all oxidized products or specifically hydrogen peroxide?

    1 answer

    1. MAK165 is used to quantify hydrogen peroxide in solutions and cell extracts. Therefore, the kit is specific for hydrogen peroxide and does not measure other oxidized products.

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  6. Are black plates with black bottoms suitable for use with the MAK165 kit, even though the Technical Bulletin recommends black plates with clear bottoms?

    1 answer

    1. A solid black plate or a black wall with a clear bottom plate is suitable for use.

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  7. Is the red peroxidase substrate in kit MAK165 the chemical 10-acetyl-3,7-dihydroxyphenoxazine (also known as Amplex Red)?

    1 answer

    1. The red peroxidase substrate in kit MAK165 is Amplex Red, which is the trademark of Invitrogen. The substrate is 10-Acetyl-3,7-dihydroxyphenoxazine and is chemically the same.

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