The fluorescent sensor in the MAK164-1KT Intracellular Hydrogen Peroxide Assay is fluorescein-based. The sensor initially exists in the non-fluorescent lactone form, which is then converted into the fluorescent quinoid form upon reaction with hydrogen peroxide. The oxidation of hydrogen peroxide removes the lactone-locking group.
MAK164
Intracellular Hydrogen Peroxide Assay
sufficient for 200 fluorometric tests (green fluorescence)
Synonym(s):
Hydrogen Peroxide Detection in Cells
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usage
sufficient for 200 fluorometric tests (green fluorescence)
detection method
fluorometric
relevant disease(s)
aging/geriatric diseases; neurological disorders; pulmonary disorders; orthopedic diseases; cardiovascular diseases
storage temp.
−20°C
General description
Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.
Application
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
This kit is suitable for the detection of hydrogen peroxide in living cells and a variety of samples such as cell extracts and liquids.
Principle
The Intracellular Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in living cells and in a variety of other samples such as cellular extracts. This kit utilizes a unique cell-permeable sensor that generates a fluorescent product (λex = 490/λem = 520 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader, flow cytometer, or fluorescent microscope.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
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What is the specific reaction of the fluorescent sensor in the MAK164-1KT Intracellular Hydrogen Peroxide Assay?
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