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MABE1006

Sigma-Aldrich

Anti-N6-methyladenosine Antibody (m6A), clone 17-3-4-1

clone 17-3-4-1, from mouse

Synonym(s):

N6-methyladenosine, m6A

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

17-3-4-1, monoclonal

species reactivity (predicted by homology)

all

technique(s)

RIP: suitable
dot blot: suitable

isotype

IgG1λ

shipped in

wet ice

target post-translational modification

unmodified

General description

N6-Methyladenosine (m6A ) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment. Fat mass and obesity-associated (FTO) and ALKBH5 are two identified demethylases that catalyze oxidative demthylation of m6A. Several functions of m6A modification have been proposed, including mRNA decay, splicing regulation, RNA nuclear export and maturation. (PMID 25171402). Cat. No. MABE1006, Anti-N6-methyladenosine (m6A), clone 17-3-4-1, is a mouse monoclonal that specifically detects RNA molecules with m6A modication.

Immunogen

Epitope: N6-methyladenosine
Hapten N6-methyladenosine-5′-mono-phosphate conjugated to BSA of all N6-methyladenosine (m6A).

Application

(RNA) Immunoprecipitation Analysis: A representative lot immunoprecipitated N6-methyladenosine in IME2 transcripts (Bodi, Z., et al. (2010). Nucleic Acids Research. 38(16):5327-5335).
RNA Dot Blot Analysis: A representative lot detected N6-methyladenosine in m6A (Bodi, Z., et al. (2010). Nucleic Acids Research. 38(16):5327-5335).
Anti-N6-methyladenosine Antibody (m6A), clone 17-3-4-1 is an antibody against N6-methyladenosine for use in Dot Blot and RNA binding protein immunoprecipitation.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors

Quality

Evaluated by RNA Dot Blot in oligomers containing N6-methyladenosine.

RNA Dot Blot Analysis: 1.0-2.0 μg/mL of this antibody detected N6-methyladenosine in oligomers containing N6-methyladenosine.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1λ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Rong-Zhang He et al.
Cancer cell international, 21(1), 616-616 (2021-11-24)
UCA1 is frequently upregulated in a variety of cancers, including CRC, and it can play an oncogenic role by various mechanisms. However, how UCA1 is regulated in cancer is largely unknown. In this study, we aimed to determine whether RNA
Kang Wei et al.
Bioengineered, 13(3), 5236-5250 (2022-02-15)
N6-methyladenosine (m6A) is one of the most significant modifications in human mRNAs. Emerging evidence indicates that m6A participates in the initiation and development of malignant tumors. Nevertheless, the biological roles and mechanism of m6A in osteosarcoma (OS) remain unclear. The
Nate J Fry et al.
Oncotarget, 9(58), 31231-31243 (2018-08-23)
The mRNA modification N6-methyladenosine (m6A) is involved in many post-transcriptional regulatory processes including mRNA stability and translational efficiency. However, it is also imperative to correlate these processes with phenotypic outputs during cancer progression. Here we report that m6A levels are
Dan Jin et al.
Journal of hematology & oncology, 12(1), 135-135 (2019-12-11)
METTL3 is an RNA methyltransferase that mediates m6A modification and is implicated in mRNA biogenesis, decay, and translation. However, the biomechanism through which METTL3 regulates MALAT1-miR-1914-3p-YAP axis activity to induce NSCLC drug resistance and metastasis is not very clear. The
Junfei Han et al.
Frontiers in cell and developmental biology, 9, 659228-659228 (2022-02-11)
Glioma is the most common type of malignant tumor of the nervous system and is characterized by high mortality and poor outcome. This study aims to investigate the mechanism underlying IGF2 mRNA-binding protein 2 (IGF2BP2) and long noncoding RNA DANCR

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