์ฝ˜ํ…์ธ ๋กœ ๊ฑด๋„ˆ๋›ฐ๊ธฐ
Merck
  • A sensitive and efficient method for trace analysis of some phenolic compounds using simultaneous derivatization and air-assisted liquid-liquid microextraction from human urine and plasma samples followed by gas chromatography-nitrogen phosphorous detection.

A sensitive and efficient method for trace analysis of some phenolic compounds using simultaneous derivatization and air-assisted liquid-liquid microextraction from human urine and plasma samples followed by gas chromatography-nitrogen phosphorous detection.

Biomedical chromatography : BMC (2015-05-28)
Mir Ali Farajzadeh, Mohammad Reza Afshar Mogaddam, Ali Akbar Alizadeh Nabil
์ดˆ๋ก

In present study, a simultaneous derivatization and air-assisted liquid-liquid microextraction method combined with gas chromatography-nitrogen phosphorous detection has been developed for the determination of some phenolic compounds in biological samples. The analytes are derivatized and extracted simultaneously by a fast reaction with 1-flouro-2,4-dinitrobenzene under mild conditions. Under optimal conditions low limits of detection in the range of 0.05-0.34 ng mL(-1) are achievable. The obtained extraction recoveries are between 84 and 97% and the relative standard deviations are less than 7.2% for intraday (n = 6) and interday (n = 4) precisions. The proposed method was demonstrated to be a simple and efficient method for the analysis of phenols in biological samples.

MATERIALS
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๋ธŒ๋žœ๋“œ
์ œํ’ˆ ์„ค๋ช…

Supelco
1,2-Dibromoethane solution, certified reference material, 200 ฮผg/mL in methanol
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Supelco
Phenol solution, certified reference material, 500 ฮผg/mL in methanol
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Supelco
Phenol solution, 5000 ฮผg/mL in methanol, certified reference material
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Sigma-Aldrich
2-Ethylpyridine, 97%
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Sigma-Aldrich
Pyridine, anhydrous, 99.8%
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Sigma-Aldrich
Phenol, unstabilized, ReagentPlusยฎ, โ‰ฅ99%
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Sigma-Aldrich
1,1,2-Trichloroethane, contains โ‰ค3% 2-propanol as stabilizer, 97%
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Sigma-Aldrich
Phenol, ACS reagent, โ‰ฅ99.0%
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Sigma-Aldrich
Phenol, โ‰ฅ96.0% (calc. on dry substance, T)
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Sigma-Aldrich
2-Naphthol, 99%
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Sigma-Aldrich
m-Cresol, โ‰ฅ98%, FG
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Sigma-Aldrich
Phenol, unstabilized, purified by redistillation, โ‰ฅ99%
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Sigma-Aldrich
2-Methylpyridine, 98%
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Sigma-Aldrich
2-Naphthol, 98%
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Sigma-Aldrich
Pyridine, โ‰ฅ99%
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Sigma-Aldrich
Phenol, puriss. p.a., ACS reagent, reag. Ph. Eur., 99.0-100.5%
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Sigma-Aldrich
Phenol, BioXtra, โ‰ฅ99.5% (GC)
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Sigma-Aldrich
Liquified Phenol, โ‰ฅ89.0%
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Sigma-Aldrich
Phenol, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.5-100.5% (GC)
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Sigma-Aldrich
Phenol, โ‰ฅ99%
๊ฐ€๊ฒฉ ๋ฐ ์žฌ๊ณ  ์ •๋ณด๋ฅผ ํ˜„์žฌ ์ด์šฉํ•  ์ˆ˜ ์—†์Œ
Sigma-Aldrich
Phenol, puriss., โ‰ฅ99.5% (GC), meets analytical specification of Ph. Eur., BP, USP, crystalline (detached)
๊ฐ€๊ฒฉ ๋ฐ ์žฌ๊ณ  ์ •๋ณด๋ฅผ ํ˜„์žฌ ์ด์šฉํ•  ์ˆ˜ ์—†์Œ
Sigma-Aldrich
Pyridine, puriss. p.a., ACS reagent, reag. Ph. Eur., โ‰ฅ99.5% (GC)
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Sigma-Aldrich
Methanol solution, NMR reference standard, 4% in methanol-d4 (99.8 atom % D), NMR tube size 3 mm ร— 8 in.
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Sigma-Aldrich
Phenol, for molecular biology
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Sigma-Aldrich
Phenol solution, BioReagent, Saturated with 0.01 M citrate buffer, pH 4.3 ยฑ 0.2, for molecular biology
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Sigma-Aldrich
Phenol, BioUltra, for molecular biology, TE-saturated, ~73% (T)
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Sigma-Aldrich
Phenol solution, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology
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Supelco
Methanol solution, contains 0.10 % (v/v) formic acid, UHPLC, suitable for mass spectrometry (MS), โ‰ฅ99.5%
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Sigma-Aldrich
Methanol-12C, 99.95 atom % 12C
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Sigma-Aldrich
Pyridine, ReagentPlusยฎ, โ‰ฅ99%
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