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  • Microencapsulation of dopamine neurons derived from human induced pluripotent stem cells.

Microencapsulation of dopamine neurons derived from human induced pluripotent stem cells.

Biochimica et biophysica acta (2014-10-05)
Shuhei Konagaya, Hiroo Iwata
초록

Dopamine neurons derived from induced pluripotent stem cells have been widely studied for the treatment of Parkinson's disease. However, various difficulties remain to be overcome, such as tumor formation, fragility of dopamine neurons, difficulty in handling large numbers of dopamine neurons, and immune reactions. In this study, human induced pluripotent stem cell-derived precursors of dopamine neurons were encapsulated in agarose microbeads. Dopamine neurons in microbeads could be handled without specific protocols, because the microbeads protected the fragile dopamine neurons from mechanical stress. hiPS cells were seeded on a Matrigel-coated dish and cultured to induce differentiation into a dopamine neuronal linage. On day 18 of culture, cells were collected from the culture dishes and seeded into U-bottom 96-well plates to induce cell aggregate formation. After 5 days, cell aggregates were collected from the plates and microencapsulated in agarose microbeads. The microencapsulated aggregates were cultured for an additional 45 days to induce maturation of dopamine neurons. Approximately 60% of all cells differentiated into tyrosine hydroxylase-positive neurons in agarose microbeads. The cells released dopamine for more than 40 days. In addition, microbeads containing cells could be cryopreserved. hiPS cells were successfully differentiated into dopamine neurons in agarose microbeads. Agarose microencapsulation provides a good supporting environment for the preparation and storage of dopamine neurons.

MATERIALS
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브랜드
제품 설명

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Purmorphamine, ≥98% (HPLC)
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DL-Tyrosine, 99%
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Ascorbic acid, European Pharmacopoeia (EP) Reference Standard
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Sodium trichloroacetate, 97%
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2-Mercaptoethanol, BioUltra, for molecular biology, ≥99.0% (GC)
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2-Mercaptoethanol, ≥99.0%
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2-Mercaptoethanol, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
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