63689

2-Mercaptoethanol

BioUltra, for molecular biology, ≥99.0% (GC)

• 동의어(들): β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
• Linear Formula: HSCH₂CH₂OH
• CAS Number: 60-24-2
• Molecular Weight: 78.13
• Beilstein: 773648
• EC Number: 200-464-6
• MDL number: MFCD00004890
• UNSPSC 코드: 12161504
• PubChem Substance ID: 57651953
• NACRES: NA.25

속성

• Grade: for molecular biology
• Quality Level: 200
• vapor density: 2.69 (vs air)
• vapor pressure: 1 mmHg ( 20 °C)
• 제품 라인: BioUltra
• 분석: ≥99.0% (GC)
• 형태: liquid
• expl. lim.: 18 %
• 반응 적합성: reagent type: reductant
• 농도: 14.3 M (pure liquid)
• 기술: RNA extraction: suitable; protein extraction: suitable
• 불순물: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• refractive index: n20/D 1.500 (lit.); n20/D 1.501
• pH: 4.5-6 (20 °C, 500 g/L)
• bp: 157 °C (lit.)
• solubility: H₂O: 0.5 M at 20 °C, clear, colorless
• density: 1.114 g/mL at 25 °C (lit.)
• 양이온 미량물: Al: ≤0.5 mg/kg; Ba: ≤0.1 mg/kg; Bi: ≤0.1 mg/kg; Ca: ≤0.5 mg/kg; Cd: ≤0.05 mg/kg; Co: ≤0.02 mg/kg; Cr: ≤0.02 mg/kg; Cu: ≤0.02 mg/kg; Fe: ≤0.1 mg/kg; K: ≤0.5 mg/kg; Li: ≤0.1 mg/kg; Mg: ≤0.1 mg/kg; Mn: ≤0.02 mg/kg; Mo: ≤0.1 mg/kg; Na: ≤1 mg/kg; Ni: ≤0.05 mg/kg; Pb: ≤0.1 mg/kg; Sr: ≤0.1 mg/kg; Zn: ≤0.1 mg/kg
• SMILES string: OCCS
• λ: 0.5 M in H₂O
• UV 흡수: λ: 260 nm A_max: 1.5; λ: 280 nm A_max: 0.3
• 저장 온도: 2-8°C
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• InChI key: DGVVWUTYPXICAM-UHFFFAOYSA-N

설명

애플리케이션

2-Mercaptoethanol has been used

• in the protein extraction buffer prepared for leaf samples.
• as a supplement in cell culture media.
• in the procedure of RNA extraction.

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds

안전성 정보

• 픽토그램: GHS06,GHS08,GHS05,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H301 + H331,H310,H315,H317,H318,H361f,H373,H410
• 예방조치 성명서: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
• 표적 기관: Liver,Heart
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point (°F): 165.2 °F - closed cup
• Flash Point (°C): 74 °C - closed cup
• 개인 보호 장비: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter