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M3148

Sigma-Aldrich

2-Mercaptoethanol

for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

동의어(들):

β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

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크기 선택

25 ML
₩51,156
100 ML
₩146,871
250 ML
₩210,308
500 ML
₩376,667

₩51,156

재고 있음세부사항

벌크 견적 요청
모든 사진(3)

크기 선택

보기 변경
25 ML
₩51,156
100 ML
₩146,871
250 ML
₩210,308
500 ML
₩376,667

About This Item

Linear Formula:
HSCH2CH2OH
CAS Number:
60-24-2
Molecular Weight:
78.13
Beilstein:
773648
EC Number:
200-464-6
MDL number:
MFCD00004890
UNSPSC 코드:
12161504
PubChem Substance ID:
24896800
NACRES:
NA.31

₩51,156

재고 있음세부사항

벌크 견적 요청

Grade

for molecular biology

Quality Level

200

vapor density

2.69 (vs air)

vapor pressure

1 mmHg ( 20 °C)

제품 라인

BioReagent

분석

99% (GC/titration)

양식

liquid

expl. lim.

18 %

반응 적합성

reagent type: reductant

농도

14.3 M (pure liquid)

기술

cell culture | mammalian: suitable
electrophoresis: suitable

refractive index

n20/D 1.500 (lit.)

pH

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

solubility

H2O: soluble 1 mL/mL

density

1.114 g/mL at 25 °C (lit.)

외래 활성

DNase, RNase, protease, none detected

저장 온도

2-8°C

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

유사한 제품을 찾으십니까? 방문 제품 비교 안내

애플리케이션

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
Reducing agent
β-mercaptoethanol or 2-mercaptoethanol has been used:
  • as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line)[1]
  • to lyse NuLi-1 (Normal Lung, University of Iowa-1) cells[2]
  • to trypsinize mouse embryonic stem cells[3]

신호어

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

표적 기관

Liver,Heart

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point (°F)

154.4 °F - closed cup

Flash Point (°C)

68 °C - closed cup

개인 보호 장비

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
BCCM6691
0000385093
0000385092
0000385093
0000385092

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COA 검색

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Judy J
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In embryonic stem (ES) cells, bivalent chromatin domains with overlapping repressive (H3 lysine 27 tri-methylation) and activating (H3 lysine 4 tri-methylation) histone modifications mark the promoters of more than 2,000 genes. To gain insight into the structure and function of
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프로토콜

Protocol Guide: XTT Assay for Cell Viability and Proliferation

XTT assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for XTT reagent preparation and examples of applications.

MTT Assay Protocol for Cell Viability and Proliferation

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

세포 생존성 및 증식을 위한 MTT 분석 프로토콜

세포 생존성, 증식, 세포독성 측정을 위한 MTT 분석 프로토콜. MTT 시약 조제에 대한 지침 및 응용분야 예시.

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

질문

1–5 / 5 질문  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 답변

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 답변

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. I’ve purchased 25mL bottle and want to use this in my cell culture media at concentration of 50mM. What is the concentration in the 25mL bottle and how to make this to 50mM concentration?

    1 답변

    1. This product is a neat or pure material. The concentration is considered to be 100%. The calculated molarity is 14.3 M as reported in the Properties section of the product page.
      To prepare a 50 mM solution, please mix 0.391 g of M3148 with 100 mL solvent. 0.391 g / 78.13 g/mol / 0.1 L = 0.05 = 50 mM.

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  4. Hi, What is the concentration of 2-mercaptoethanol in the 100mL bottle? 

    1 답변

    1. This product is a neat or pure material. The concentration is considered to be 100%. The calculated molarity is 14.3 M as reported in the Properties section of the product page.

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  5. Hi is this sterile?

    1 답변

    1. This product is not sterile, but can be used in cell culture applications and is DNase, RNase, and protease free.

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