A6877

Agarose

Medium EEO, for molecular biology

• 동의어(들): 3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose ME
• CAS Number: 9012-36-6
• EC Number: 232-731-8
• MDL number: MFCD00081294
• UNSPSC 코드: 41105317
• PubChem Substance ID: 24891148
• NACRES: NA.25

속성

• 생물학적 소스: algae (marine)
• Quality Level: 200
• Grade: for molecular biology
• 유형: Type II
• 형태: powder
• 불순물: ≤10% moisture content
• EEO: 0.16-0.19
• 전이 온도: congealing temperature 34.5-37.5 °C (1.5% gel)
• 겔 강도: ≥1000 g/cm² (1.0% gel)
• InChI: 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
• InChI key: MJQHZNBUODTQTK-WKGBVCLCSA-N

설명

일반 설명

Agarose is a polymer extracted from agar or agar-bearing marine algae. This purified linear galactan hydrocolloid comprises alternating co-polymers D-galactose and 3,6-anhydro-L-galactose units connected by α-(1→3) and β-(1→4) glycosidic bonds. Agarose is highly biocompatible, possesses variable mechanical and diffusion properties.

애플리케이션

Agarose has been used:

• in gel electrophoresis
• in the preparation of 2x agarose solution used along with 2x supplemented-MEM media as an overlay medium in plaque assay
• in an immunodiffusion test for Paracoccidioidomycosis

분석 메모

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)