567513-U

Discovery^® HS F5 (5 µm) HPLC Columns

L × I.D. 5 cm × 4.6 mm, HPLC Column

• 동의어(들): Discovery Pentafluorophenyl PFP HPLC Column
• UNSPSC 코드: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

속성

• 제품명: Discovery^® HS F5 HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L43
• 제품 라인: Discovery^®
• 특징: endcapped
• 제조업체/상표: Discovery^®
• 포장: 1 ea of
• 라벨링 범위: 12% Carbon loading
• 파라미터: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• 기술: HPLC: suitable; LC/MS: suitable
• 길이 × I.D.: 5 cm × 4.6 mm
• 표면적: 300 m²/g
• 표면 범위: 4 μmol/m²
• 불순물: <10 ppm metals
• Matrix: silica gel, high purity, spherical particle platform; fully porous particle
• 기질 활성군: PFP (pentafluorophenyl) phase
• 입자 크기: 5 μm
• 공극 크기: 120 Å
• 작동 pH 범위: 2-8
• 응용 분야: food and beverages
• 분리 기술: reversed phase

설명

일반 설명

Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log P_o/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.

The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

애플리케이션

• Optimization and validation of a bioanalytical HPLC-UV technique for simultaneous determination of underivatized phenylalanine and tyrosine in the blood for phenylketonuria diagnosis and monitoring.: This study utilizes the Discovery^® HS F5 HPLC Column to develop an HPLC-UV method for the simultaneous determination of phenylalanine and tyrosine in blood, significantly advancing the diagnostic and monitoring capabilities for phenylketonuria (Rabti et al., 2024).


• A new RP-HPLC method as an auxiliary tool for optimization of sample preparation procedures for tracing of PPCPs of different hydrophilicities.: Demonstrates the application of the Discovery^® HS F5 HPLC Column in optimizing sample preparation techniques for the detection of pharmaceuticals and personal care products in environmental samples, enhancing the analysis of diverse hydrophilic compounds (Portillo-Castillo et al., 2021).


• "Heart-cut" bidimensional achiral-chiral liquid chromatography applied to the evaluation of stereoselective metabolism, in vivo biological activity and brain response to chiral drug candidates targeting the central nervous system.: Utilizes the Discovery^® HS F5 HPLC Column in a novel bidimensional chromatographic setup to study the stereoselective metabolism of CNS-targeting chiral drugs, contributing to a deeper understanding of their pharmacological profiles (Battisti et al., 2016).


• HPLC method development, validation, and impurity characterization of a potent antitumor indenoisoquinoline, LMP776 (NSC 725776).: This research employs the Discovery^® HS F5 HPLC Column for developing an HPLC method to identify and characterize impurities in the antitumor drug LMP776, ensuring drug purity and compliance with regulatory standards (Wang et al., 2016).


• Development studies on determination of preservatives decomposition products.: Showcases the application of the Discovery^® HS F5 HPLC Column in the analysis of decomposition products of preservatives, enhancing the safety assessments of food and pharmaceutical products (Zabrzewska et al., 2014).

특징 및 장점

• Unique selectivity
• Similar retention to C18 (sometimes requires stronger mobile phase)
• Excellent peak shape
• Stable, low-bleed LC-MS separations
• Scalable separations from 3 to 10μm particle sizes

추천 제품

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

법적 정보

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany