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Merck
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문서

567517-U

Supelco

Discovery® HS F5 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

동의어(들):

Discovery Pentafluorophenyl PFP HPLC Column

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About This Item

UNSPSC 코드:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Discovery® HS F5 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

material

stainless steel column

Agency

suitable for USP L43

제품 라인

Discovery®

특징

endcapped

제조업체/상표

Discovery®

포장

1 ea of

라벨링 범위

12% Carbon loading

파라미터

≤70 °C temp. range
400 bar pressure (5801 psi)

기술

HPLC: suitable
LC/MS: suitable

길이 × I.D.

25 cm × 4.6 mm

표면적

300 m2/g

표면 범위

4 μmol/m2

불순물

<10 ppm metals

기질

silica gel, high purity, spherical particle platform
fully porous particle

기질 활성군

PFP (pentafluorophenyl) phase

입자 크기

5 μm

공극 크기

120 Å

operating pH range

2-8

응용 분야

food and beverages

분리 기술

reversed phase

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관련 카테고리

일반 설명

Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

애플리케이션


  • Determination of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline, 5-hydroxytryptamine and 5-hydroxyindole acetic acid in the neonatal rat brains using high performance liquid chromatography-electrochemical detection.: This study highlights the use of the Discovery® HS F5 HPLC Column for the sensitive detection of neuroactive compounds in neonatal rat brain samples, advancing our understanding of neurological development and neurotransmitter dynamics (Mao et al., 2009).

  • Advantages of pentafluorophenylpropyl stationary phase over conventional C18 stationary phase--application to analysis of triamcinolone acetonide.: This research showcases the superior performance of the Discovery® HS F5 HPLC Column in analyzing corticosteroid formulations, providing insights that could lead to improved analytical methodologies in pharmaceutical analysis (Havlikova et al., 2008).

  • Determination of ephedrine alkaloids in Ephedra natural products using HPLC on a pentafluorophenylpropyl stationary phase.: Utilizes the Discovery® HS F5 HPLC Column for the precise and efficient separation of ephedrine alkaloids, contributing to safer and more effective use of Ephedra in herbal supplements (Pellati and Benvenuti, 2008).

  • Fast high-performance liquid chromatography analysis of phenethylamine alkaloids in Citrus natural products on a pentafluorophenylpropyl stationary phase.: Demonstrates the efficiency of the Discovery® HS F5 HPLC Column in the rapid analysis of phenethylamine alkaloids, enhancing the quality control of Citrus-based dietary supplements (Pellati and Benvenuti, 2007).

  • High-performance liquid chromatographic method with UV photodiode-array, fluorescence and mass spectrometric detection for simultaneous determination of galantamine and its phase I metabolites in biological samples.: This study uses the Discovery® HS F5 HPLC Column to simultaneously quantify galantamine and its metabolites, aiding in the pharmacokinetic profiling of this Alzheimer′s disease medication (Malakova et al., 2007).

특징 및 장점

  • Unique selectivity
  • Similar retention to C18 (sometimes requires stronger mobile phase)
  • Excellent peak shape
  • Stable, low-bleed LC-MS separations
  • Scalable separations from 3 to 10μm particle sizes

추천 제품

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

법적 정보

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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문서 라이브러리 방문

Koji Baba et al.
Journal of chromatography. A, 1354, 109-116 (2014-06-21)
Arsenic species, including arsenous acid, arsenic acid, methylarsonic acid, and dimethylarsinic acid, were determined using HPLC-ICPMS. The species were separated with a Discovery HS F5 column and a simple, volatile, and isocratic mobile phase of 0.1% (v/v) formic acid and
Ilia Brondz et al.
Journal of pharmaceutical and biomedical analysis, 43(3), 937-944 (2006-11-03)
The drug primaquine diphosphate is used for causative treatment of malaria. Using HPLC-MS and GC-MS, this research group was previously able to show that the main contaminant of primaquine is the positional isomer quinocide [I. Brondz, D. Mantzilas, U. Klein
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 48(2), 254-263 (2007-12-14)
In this study a pentafluorophenylpropyl (PFPP) stationary phase was applied to the fast and reliable qualitative and quantitative analysis of ephedrine alkaloids in Ephedra plant material and derivatives. A Discovery HS F5 column (150mmx4.6mm i.d., 5microm) was used, with an
Federica Pellati et al.
Journal of chromatography. A, 1165(1-2), 58-66 (2007-08-07)
In this study, the chromatographic performance of a pentafluorophenylpropyl (PFPP) stationary phase was evaluated for the rapid separation of phenethylamine alkaloids (i.e. (+/-)-octopamine, (+/-)-synephrine, tyramine, N-methyltyramine and hordenine) in Citrus aurantium plant material (fruits and peel), various Citrus species, extracts
Umberto M Battisti et al.
Journal of chromatography. A, 1443, 152-161 (2016-03-30)
A "heart-cut" two-dimensional achiral-chiral liquid chromatography triple-quadrupole mass spectrometry method (LC-LC-MS/MS) was developed and coupled to in vivo cerebral microdialysis to evaluate the brain response to the chiral compound (±)-7-chloro-5-(3-furanyl)-3-methyl-3,4-dihydro-2H-1,2,4-benzothiadiazine-1,1-dioxide ((±)-1), a potent positive allosteric modulator (PAM) of AMPA receptor.

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