콘텐츠로 건너뛰기
Merck
모든 사진(1)

주요 문서

53313-U

Supelco

Ascentis® Express Peptide 160 Å ES-C18 (2.7 μm) HPLC Columns

L × I.D. 10 cm × 3 mm, HPLC Column

로그인조직 및 계약 가격 보기

크기 선택

1 EA
₩1,268,344

₩1,268,344

출고 가능일2025년 4월 11일세부사항

모든 사진(1)

크기 선택

보기 변경
1 EA
₩1,268,344

About This Item

UNSPSC 코드:
41115700
eCl@ss:
32110501
NACRES:
SB.52

₩1,268,344

출고 가능일2025년 4월 11일세부사항

제품명

Ascentis® Express Peptide ES-C18, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 10 cm × 3 mm

material

stainless steel column

Agency

suitable for USP L1

제품 라인

Ascentis®

특징

endcapped: no

제조업체/상표

Ascentis®

포장

1 ea of

파라미터

≤100 °C temp. range
600 bar max. pressure (9000 psi)

기술

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

길이 × I.D.

10 cm × 3 mm

표면적

90 m2/g

불순물

<5 ppm metals

Matrix

Fused-Core particle platform
superficially porous particle

기질 활성군

C18 (octadecyl) phase

입자 크기

2.7 μm

공극 크기

160 Å

작동 pH 범위

1-9

분리 기술

reversed phase

유사한 제품을 찾으십니까? 방문 제품 비교 안내

관련 카테고리

일반 설명

Ascentis Express Peptide ES-C18 columns are specifically engineered to separate higher molecular weight compounds such as peptides and small proteins. These columns contain advanced Fused-Core particles that have larger pores (160 Å versus 90 Å in standard Ascentis Express), bonded with sterically-protected C18 ligands to provide extra stability (ES) at very low pH (< 1) and high temperatures (up to 100ºC). This greatly expands the application range for Ascentis Express columns.

법적 정보

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

가아드 카트리지

제품 번호
설명
가격

관련 제품

제품 번호
설명
가격

애플리케이션

제품 번호
설명
가격

필요하나 제공되지 않음

제품 번호
설명
가격

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
USUM001358
USUM001357
USUM001356
USUM001354
USUM001344

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Meiyun Shi et al.
Journal of separation science, 38(8), 1351-1357 (2015-01-30)
The pentapeptide thymopentin (Arg-Lys-Asp-Val-Tyr, RKDVY) corresponds to amino acids 32-36 of the 49 amino acid immunomodulatory polypeptide, thymopoietin, whose biological activity is partially reproduced. Thymopentin is widely used in the clinic and represents a promising target for drug design but
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
C Gröer et al.
Journal of pharmaceutical and biomedical analysis, 100, 393-401 (2014-09-15)
Cytochrome P450 (CYP) enzymes and UDP-glucuronosyltransferases (UGT) are major determinants in the pharmacokinetics of most drugs on the market. To investigate their impact on intestinal and hepatic drug metabolism, we developed and validated quantification methods for nine CYP (CYP1A2, CYP2B6

문서

Ascentis® Express Peptide ES-C18 U/HPLC Columns

Choose Ascentis® Express Peptide ES-C18 U/HPLC Columns based on Fused-Core® technology for fast and efficient separation of high-molecular weight compounds, such as peptides and small proteins. With a 2.7 µm particle size and rigorous testing, these columns offer reliable results and high efficiency.

Chromatograms

application for HPLC

질문

1–10 / 12 질문  

  1. Can product 53313-U Ascentis® Express Peptide ES C18 HPLC column be used for LC-MS?

    1 답변

    1. Yes, Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of product 53313-U Ascentis® Express Peptide ES C18 HPLC Column for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.

      도움이 되었습니까?

  2. Can I use product 53313-U Ascentis® Express Peptide ES C18 HPLC Column on any type of HPLC system?

    1 답변

    1. Product 53313-U Ascentis® Express Peptide ES C18 HPLC Column HPLC columns is capable of use on standard HPLC systems as well as UHPLC systems.  This column is packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.

      도움이 되었습니까?

  3. Do I need special fittings and tubing to connect product 53313-U Ascentis® Express Peptide ES C18 HPLC ColumnExpress columns?

    1 답변

    1. While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.

      도움이 되었습니까?

  4. Are guard columns available for product 53313-U Ascentis® Express Peptide ES C18 HPLC Column?

    1 답변

    1. Yes, we have guard columns available for product 53313-U Ascentis® Express Peptide ES C18 HPLC Column.  Product 53500-U is the Ascentis® Express Guard Holder and Product 53537-U Ascentis® Express Peptide ES C18 Guard Cartridge is a package of three guard cartridges, 2.7 μm particle size, L 5mm × I.D. 3.0 mm .

      도움이 되었습니까?

  5. What is the carbon load for product 53313-U Ascentis® Express Peptide ES C18 HPLC Column?

    1 답변

    1. Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles.  The carbon coverage for product 53313-U Ascentis® Express Peptide ES C18 HPLC column is 2 micromoles/meter-squared.

      도움이 되었습니까?

  6. Is there anything special I need to do to my HPLC system to use product 53313-U Ascentis® Express Peptide ES C18 HPLC Column?

    1 답변

    1. Nothing special is required to use product 53313-U Ascentis® Express Peptide ES C18 HPLC Column. To obtain the full benefits of Ascentis® Express Column, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Performance with Ascentis® Express HPLC Columns (T407102).

      도움이 되었습니까?

  7. What is special about product 53313-U Ascentis® Express Peptide ES C18 HPLC Column?

    1 답변

    1. Product 53313-U Ascentis® Express Peptide ES C18 HPLC Column provides a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis® Express columns provide the benefits of sub-2 μm particles but at much lower backpressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

      도움이 되었습니까?

  8. What is the pressure rating for product 53313-U Ascentis® Express Peptide ES C18 HPLC Column?

    1 답변

    1. This product 53313-U Ascentis® Express Peptide ES C18 HPLC Column is stable to operating pressures up to 600 bar (9000 psi)

      도움이 되었습니까?

  9. How can I measure my instrument bandwidth (IBW) and determine what columns can be used with minimal efficiency loss created by too much internal instrument volume?

    1 답변

    1. The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.

      도움이 되었습니까?

  10. Can I use product 53313-U Ascentis® Express Peptide ES C18 HPLC Column on a UHPLC system?

    1 답변

    1. Yes.  product 53313-U Ascentis® Express Peptide ES C18 HPLC columns are packed in a way making them suitable for these ultra high pressure instruments.  In fact, product 53313-U Ascentis® Express Peptide ES C18 HPLC Column outperforms sub-2 μm micron columns on many applications since product 53313-U Ascentis® Express Peptide ES C18 HPLC Column provides the benefits of sub-2 μm particles but at much lower back pressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

      도움이 되었습니까?

1–10 / 12 질문  

후기

평점 값 없음

활성 필터

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.