The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
₩918,778
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추천 제품
제품명
Ascentis® Express C18, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 5 cm × 4.6 mm
material
stainless steel column
Quality Level
Agency
suitable for USP L1
제품 라인
Ascentis®
특징
endcapped
제조업체/상표
Ascentis®
포장
1 ea of
파라미터
60 °C temp. range
600 bar max. pressure (9000 psi)
기술
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
길이 × I.D.
5 cm × 4.6 mm
표면적
135 m2/g
불순물
<5 ppm metals
Matrix
Fused-Core particle platform
superficially porous particle
기질 활성군
C18 (octadecyl) phase
입자 크기
2.7 μm
공극 크기
90 Å
작동 pH
2-9
응용 분야
food and beverages
분리 기술
reversed phase
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users.
Visit the Ascentis Express home page for more information on this new column technology.
Visit the Ascentis Express home page for more information on this new column technology.
애플리케이션
법적 정보
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters
가아드 카트리지
관련 제품
애플리케이션
제품 번호
설명
가격
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
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문서
Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection.
An RP-LC×RP-LC-PDA-MS method for the metabolic profiling of G. glabra extract using the MSG approach for enhanced metabolic coverage.
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관련 콘텐츠
Accurately detect and quantify trace nitrosamines (NDMA, NDEA, NEIPA, NDIPA, NDBA, etc.) in pharmaceutical drugs using our complete product portfolio and application guides for LC-MS and GC-MS. Order high-quality reference standards, columns, filters & more.
Chromatograms
application for HPLC, application for SPEapplication for HPLC, application for SPEapplication for HPLCapplication for HPLC-
How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?
1 답변-
도움이 되었습니까?
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What flow rate should I use with Ascentis® Express HPLC Columns?
1 답변-
Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
도움이 되었습니까?
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What is the Department of Transportation shipping information for this product?
1 답변-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
도움이 되었습니까?
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Can Ascentis® Express HPLC Columns be used for LC-MS?
1 답변-
Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
도움이 되었습니까?
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Is there anything special I need to do to my HPLC system to use Ascentis Express?
1 답변-
Nothing special is required to use Ascentis Express HPLC columns. To obtain the full benefits of Ascentis Express, one should minimize dispersion in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102).
도움이 되었습니까?
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Can I use Ascentis Express on a UHPLC system?
1 답변-
Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
도움이 되었습니까?
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Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?
1 답변-
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
도움이 되었습니까?
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Can I use Ascentis Express on any type of HPLC system?
1 답변-
Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems. Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
도움이 되었습니까?
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What is the carbon load for Ascentis® Express C18 HPLC Columns?
1 답변-
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis® Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for Ascentis® Express C18 is 3.5 micromoles/meter-squared
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