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Merck
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505013

Supelco

Discovery® RP-Amide C16 (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

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About This Item

UNSPSC 코드:
41115700
eCl@ss:
32110501

product name

Discovery® RP-AmideC16 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

Agency

suitable for USP L60

특징

endcapped

제조업체/상표

Discovery®

라벨링 범위

11% Carbon loading

파라미터

≤70 °C temp. range
400 bar pressure (5801 psi)

기술

HPLC: suitable

길이 × I.D.

15 cm × 4.6 mm

표면적

200 m2/g

표면 범위

2.6 μmol/m2

불순물

<10 ppm metals

기질

silica gel, high purity, spherical base material

기질 활성군

amide, alkyl phase

입자 크기

5 μm

공극 크기

180 Å

operating pH range

2-8

응용 분야

food and beverages

분리 기술

reversed phase

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관련 카테고리

일반 설명

Discovery® RP-AmideC16 HPLC Column consists of a stationary phase made up of high-purity C16-silica that includes a polar-embedded amide function attached to the silica surface with a propyl group.

애플리케이션

Discovery RP-AmideC16 HPLC Column may be used for the separation of:
  • Impurities in drugs such as closantel, levamisole and azaconazole during their determination by high performance liquid chromatography-diode array detection (HPLC-DAD).
  • Vitamin B6 compounds in food samples during their determination using LC coupled with fluorescence detection (FLD).
  • Vitamin C and other fat soluble vitamins in mature seeds of Lathyrus L. taxa during their identification using HPLC with ultraviolet (UV) detection.
  • Zidovudine in combination with other pharmaceuticals in intravenous fluid mixtures during the determination using HPLC combined with photodiode array (PDA) detector.

Discovery® RP-AmideC16 HPLC column may be used for the separation of:
  • Phenolic compounds, purine alkaloids and theanine in Camellia species by high performance liquid chromatography (HPLC) equipped with photodiode array detector (PDA).
  • Proprietary pharmaceutical compounds using HPLC combined with computer-assisted screening and Plackett−Burman experimental design.
  • Hydrophilic bases by LC with principal component analysis (PCA).
  • 2,4-Dinitrophenylhydrazone derivatives by HPLC equipped with ultraviolet (UV) spectrometric detection.

특징 및 장점

  • Excellent retention and resolution of polar compounds
  • Unique selectivity compared to C18
  • Excellent peak shape and efficiency
  • Less hydrophobic than C18 columns
  • Compatible with 100% aqueous mobile phases
   

추천 제품

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

법적 정보

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

HPLC separations of zidovudine and selected pharmaceuticals using a hexadecylsilane amide column.
Caufield WV and Stewart JT
Chromatographia, 54(9-10), 561-568 (2001)
Determination of vitamin B6 compounds in foods using liquid chromatography with post-column derivatization fluorescence detection.
Vinas P, et al.
Chromatographia, 59(5-6), 381-386 (2004)
An improved HPLC method for simultaneous determination of phenolic compounds, purine alkaloids and theanine in Camellia species
Peng L, et al.
J. Food Compos. Anal., 21(7), 559-563 (2008)
Strategy for developing and optimizing liquid chromatography methods in pharmaceutical development using computer-assisted screening and Plackett-Burman experimental design
Li W and Rasmussen HT
Journal of Chromatography A, 1016(2), 165-180 (2003)
Selection of reversed-phase liquid chromatographic columns with diverse selectivity towards the potential separation of impurities in drugs.
Van Gyseghem E, et al.
Journal of Chromatography A, 1042(1-2), 69-80 (2004)

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