콘텐츠로 건너뛰기
Merck
모든 사진(1)

주요 문서

565325-U

Supelco

Ascentis® RP-Amide (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

동의어(들):

RP-Amide Reversed-Phase Chromatography Column

로그인조직 및 계약 가격 보기

크기 선택

1 EA
₩1,443,838

₩1,443,838

예상 입고일2025년 4월 28일세부사항

모든 사진(1)

크기 선택

보기 변경
1 EA
₩1,443,838

About This Item

UNSPSC 코드:
41115700
eCl@ss:
32110501
NACRES:
SB.52

₩1,443,838

예상 입고일2025년 4월 28일세부사항

제품명

Ascentis® RP-Amide HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

material

stainless steel column

Quality Level

100

Agency

suitable for USP L60

제품 라인

Ascentis®

특징

endcapped

제조업체/상표

Ascentis®

포장

1 ea of

라벨링 범위

19.5% Carbon loading

파라미터

≤70 °C temp. range
400 bar pressure (5801 psi)

기술

HPLC: suitable
LC/MS: suitable

길이 × I.D.

25 cm × 4.6 mm

표면적

450 m2/g

표면 범위

2.7 μmol/m2

불순물

<5 ppm metals

Matrix

fully porous particle
silica gel high purity, spherical

기질 활성군

amide, alkyl phase

입자 크기

5 μm

공극 크기

100 Å

작동 pH 범위

2-8

응용 분야

environmental
food and beverages

분리 기술

reversed phase

유사한 제품을 찾으십니까? 방문 제품 비교 안내

관련 카테고리

일반 설명

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.

애플리케이션


  • A validated UHPLC method for the determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts using an RP-Amide fused-core column.: This study presents a validated Ultra-High-Performance Liquid Chromatography (UHPLC) method employing an RP-Amide fused-core column for the quantitative determination of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. The method demonstrated excellent precision, accuracy, and sensitivity, making it a valuable tool for the analysis of these bioactive compounds in complex matrices. The research highlights the utility of the Ascentis® RP-Amide HPLC Column in enhancing the resolution and efficiency of chromatographic separations in analytical chemistry applications (Fibigr et al., 2018).

특징 및 장점

  • Excellent retention and peak shape for polar compounds
  • 100% aqueous compatibility
  • Ultra low bleed, LC-MS compatible
  • Unique selectivity

추천 제품

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

법적 정보

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
295054-06
295054-05
295054-04
295054-03
295054-02

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Rihana Parveen Shaik et al.
Journal of pharmaceutical analysis, 3(6), 489-499 (2013-12-01)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for simultaneous quantification of tolterodine and its metabolite 5-hydroxy methyl tolterodine in rat plasma. Tolterodine-d6 and 5-hydroxy methyl tolterodine-d14 were used as internal standards (IS). Chromatographic separation was performed on Ascentis Express
Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 151, 291-300 (2018-02-08)
The presented work describes the development and validation of a rapid UHPLC-UV method using a fused core particle column with an RP-Amide stationary phase for the separation and quantitative analysis of caffeoylquinic and di-caffeoylquinic acids in green coffee extracts. Three

질문

1–4 / 4 질문  

  1. What is the Product No of the guard column for Product No. 565325-U Ascentis RP-Amide HPLC column?

    1 답변

    1. The guard column kit for Product No. 565325-U is 565371-U. This kit includes one cartridge, stand-alone holder, a piece of tubing, 2 nuts and 2 ferrules. A set of two replacement cartridges is Product No. 565370-U.

      도움이 되었습니까?

  2. What solvent should Product No. 565325-U Ascentis RP-Amide HPLC Column  be stored in?

    1 답변

    1. Product No. 565325-U should be stored in 100% acetonitrile after any buffers have been flushed out.

      도움이 되었습니까?

  3. What is the Department of Transportation shipping information for this product?

    1 답변

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      도움이 되었습니까?

  4. Can I use Ascentis Express on a UHPLC system?

    1 답변

    1. Yes.  Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.  In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

      도움이 되었습니까?

후기

평점 값 없음

활성 필터

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.