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애플리케이션
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
성분
The supplements contain all of the following components except as indicated:
Amino acids: All standard amino acids except for leucine and tryptophan. All the amino acids are present at a concentration of 76 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L), uracil (76 mg/L).
Amino acids: All standard amino acids except for leucine and tryptophan. All the amino acids are present at a concentration of 76 mg/L.
Other nutrients: Adenine (18 mg/L), inositol (76 mg/L), p-aminobenzoic acid (8 mg/L), uracil (76 mg/L).
기타 정보
Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.
수량
1.54 g will supplement 1 liter of medium.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
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시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
Sudeshna Nag et al.
Journal of cell science, 131(18) (2018-08-30)
Sorting endosomes (SEs) are the regulatory hubs for sorting cargo to multiple organelles, including lysosome-related organelles, such as melanosomes in melanocytes. In parallel, melanosome biogenesis is initiated from SEs with the processing and sequential transport of melanocyte-specific proteins toward maturing
Riddhi Atul Jani et al.
Journal of cell science, 128(17), 3263-3276 (2015-07-26)
Melanosomes are a class of lysosome-related organelles produced by melanocytes. Biogenesis of melanosomes requires the transport of melanin-synthesizing enzymes from tubular recycling endosomes to maturing melanosomes. The SNARE proteins involved in these transport or fusion steps have been poorly studied.
문서
유전 공학 및 복제의 발전은 연구 목적을 위한 이형 Protein Expression Systems 및 분리에 수많은 가능성을 열어주었습니다. 상당한 수준의 기술 발전으로 재조합형 단백질의 대규모 발현과 분리가 가능하게 되었습니다.
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
프로토콜
Magnetic-plasmonic nanoparticles: Synthesis and application of Ag/FeCo/Ag nanobeads for cellular organelle imaging and isolation.
Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.
