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Merck
모든 사진(1)

문서

Y2021

Sigma-Aldrich

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, tryptophan, and adenine

동의어(들):

Yeast Synthetic Drop-out Media Supplements

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About This Item

MDL number:
UNSPSC 코드:
41106212
NACRES:
NA.85

Quality Level

형태

powder

기술

transformation: suitable

응용 분야

microbiology

저장 온도

room temp

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애플리케이션

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan, and adenine has been used in yeast two-hybrid screening.
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

기타 정보

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

제조 메모

Dissolve 1.39 g of supplement per liter of medium.

픽토그램

Corrosion

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Elham Alzyoud et al.
Frontiers in cell and developmental biology, 9, 727264-727264 (2021-10-19)
Microtubule nucleation in eukaryotes is primarily promoted by γ-tubulin and the evolutionary conserved protein complex, γ-Tubulin Ring Complex (γ-TuRC). γ-TuRC is part of the centrosome and basal body, which are the best-known microtubule-organizing centers. Centrosomes undergo intensive and dynamic changes
Corinne A Tovey et al.
Current biology : CB, 28(14), 2314-2323 (2018-07-10)
Microtubules are essential for various cell processes [1] and are nucleated by multi-protein γ-tubulin ring complexes (γ-TuRCs) at various microtubule organizing centers (MTOCs), including centrosomes [2-6]. Recruitment of γ-TuRCs to different MTOCs at different times influences microtubule array formation, but

프로토콜

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Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

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